| Summary: | Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Student: Lenka Böhmová Supervisor: Doc. RNDr. Miroslav Polášek, CSc. Title of Diploma Thesis: Determination of rocuronium, vecuronium and pancuronium muscle relaxants by capillary electrophoresis with contactless conductivity detection A new method of capillary zone electrophoresis with contactless conductivity detection for the determination of pancuronium bromide, vecuronium bromide and rocuronium bromide was developed. The separation was carried out in fused-silica capillary with internal diameter 50 µm, total length 75 cm and effective length 45 cm. Optimal background electrolyte was 30mM acetate buffer of pH 5,75 containing 20 mg/ml of hydroxypropyl-γ-cyclodextrin. Phenyltrimethylammonium iodide was used as internal standard. The separation was performed at 25řC, the applied voltage was 20 kV. The samples were injected hydrodynamically at 50 mbar for 6 s. Under such optimal conditions the separation took less than 4 min. Calibration curves were linear for all analytes in the range 50 - 250 µg/ml, the coefficients of correlation were in the range 0,9954 - 0,9983. The limits of detection were 13,2 µg/ml, 11,1 µg/ml and 11,3 µg/ml for pancuronium bromide, vecuronium bromide and rocuronium...
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