Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Lucie Bramborová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Supervisor specialist: RNDr. Zuzana Kročová, Ph.D. Title of Doctoral Thesis: Study of cellular and molecular mechanisms o...
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Online Access: | http://www.nusl.cz/ntk/nusl-312073 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-3120732021-03-29T05:12:24Z Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis The Study of the Cell and Molecular Mechanisms of B Cell and Intracellular Pathogen Francisella Tularensis Interaction Bramborová, Lucie Wsól, Vladimír Krejsek, Jan Šinkorová, Zuzana Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Lucie Bramborová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Supervisor specialist: RNDr. Zuzana Kročová, Ph.D. Title of Doctoral Thesis: Study of cellular and molecular mechanisms of interactions between B-cells and intracellular pathogen Francisella tularensis F. tularensis is Gram-negative, intracellular pathogen responsible for the zoonotic disease tularemia. Immunity to F. tularensis is largely mediated by T lymphocytes but an important role of B lymphocytes during early stage of infection was previously uncovered. The target host cells for F. tularensis replication are macrophages. After the entry into macrophages, F. tularensis resides in phagosomes and then escapes into the cytosol. It replicates within the cytosol and leads macrophages to apoptosis. Recently, important role of B lymphocytes in innate immunity against F. tularensis was demonstrated. Elkins et al. demonstrated strong early protection of mice that was highly dependent on B cells. Direct interaction of B lymphocytes with F. tularensis has not been described. We detected adhesion of F. tularensis to mice and human B lymphocytes, later the entry of bacteria into cells and morphological changes on mitochondria of host... 2011 info:eu-repo/semantics/doctoralThesis http://www.nusl.cz/ntk/nusl-312073 cze info:eu-repo/semantics/restrictedAccess |
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Doctoral Thesis |
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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Mgr. Lucie Bramborová Supervisor: Prof. Ing. Vladimír Wsól, Ph.D. Supervisor specialist: RNDr. Zuzana Kročová, Ph.D. Title of Doctoral Thesis: Study of cellular and molecular mechanisms of interactions between B-cells and intracellular pathogen Francisella tularensis F. tularensis is Gram-negative, intracellular pathogen responsible for the zoonotic disease tularemia. Immunity to F. tularensis is largely mediated by T lymphocytes but an important role of B lymphocytes during early stage of infection was previously uncovered. The target host cells for F. tularensis replication are macrophages. After the entry into macrophages, F. tularensis resides in phagosomes and then escapes into the cytosol. It replicates within the cytosol and leads macrophages to apoptosis. Recently, important role of B lymphocytes in innate immunity against F. tularensis was demonstrated. Elkins et al. demonstrated strong early protection of mice that was highly dependent on B cells. Direct interaction of B lymphocytes with F. tularensis has not been described. We detected adhesion of F. tularensis to mice and human B lymphocytes, later the entry of bacteria into cells and morphological changes on mitochondria of host... |
author2 |
Wsól, Vladimír |
author_facet |
Wsól, Vladimír Bramborová, Lucie |
author |
Bramborová, Lucie |
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Bramborová, Lucie Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
author_sort |
Bramborová, Lucie |
title |
Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
title_short |
Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
title_full |
Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
title_fullStr |
Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
title_full_unstemmed |
Studium buněčných a molekulárních mechanismů interakce B-buněk s intracelulárním patogenem Francisella tularensis |
title_sort |
studium buněčných a molekulárních mechanismů interakce b-buněk s intracelulárním patogenem francisella tularensis |
publishDate |
2011 |
url |
http://www.nusl.cz/ntk/nusl-312073 |
work_keys_str_mv |
AT bramborovalucie studiumbunecnychamolekularnichmechanismuinterakcebbuneksintracelularnimpatogenemfrancisellatularensis AT bramborovalucie thestudyofthecellandmolecularmechanismsofbcellandintracellularpathogenfrancisellatularensisinteraction |
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