Vývoj a validace HPLC metod pro stanovení endogenních a exogenních látek pro transportní a metabolické studie

• Main Author: Hroch, Miloš
• Other Authors: Nobilis, Milan
• Format: Doctoral Thesis
• Language: Czech
• Published: 2012
• Online Access: http://www.nusl.cz/ntk/nusl-312165

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate Ing. Miloš Hroch Consultant Doc. PharmDr. Milan Nobilis, CSc. Title of Thesis Development and validation of HPLC methods for transport and metabolic studies The presented PhD thesis deals with the development and validation of chromatographic methods in bioanalysis of drugs and their metabolites. The work provides a theoretical introduction to the field of bioanalysis, using methods of liquid chromatography. Attention was paid to recent trends in the area, particularly with respect to the new types of stationary phases. In the experimental part of the thesis, reader is introduced to a development and validation of several bio-analytical methods using high performance liquid chromatography. The first method concerns with a determination of 5-fluorouracil and its metabolites by HPLC with gradient elution and UV/VIS detection on a column Luna PFP (2). Discussed are mainly specifics for the assay of this group of polar and poorly detectable compounds. The developed and validated method is being used for TDM of patients with rectal cancer. Another discussed method deals with a determination of methotrexate polyglutamates in erythrocytes using isocratic elution on a hybrid...