Stanovení chlorbenzoových kyselin v environmentálních vzorcích pomocí separačních technik s hmotnostní detekcí

• Main Author: Hostačná, Lucie
• Other Authors: Bosáková, Zuzana
• Format: Dissertation
• Language: Czech
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-312487

Chlorobenzoic acids are a major environmental contaminant. The main source of contamination of environmental matrix is a bacterial dergadation of polychlorinated biphenyls. In this work, it has been optimized a method for the determination of 15 chlorobenzoic acid derivates (2-CBA; 3-CBA; 4-CBA; 2,3-CBA; 2,4- -CBA; 2,5-CBA; 2,6-CBA; 3,4-CBA; 3,5-CBA; 2,3,5-CBA; 2,3,6-CBA; 2,4,6- -CBA; 2,3,4,5-CBA; 2,3,5,6-CBA a 2,3,4,5,6-CBA) in the soil matrix, including accelerated solvent extraction, purification by gel permeation chromatography, derivatization and subsequent quantification of samples by GC/MS. As the extraction conditions were used: an extraction mixture of 1% acetic in hexane/acetone (1:1 V/v), temperature by 150 řC, pressure by 10,34 MPa. For purification by gel permeation chromatography solvent was used dichloromethane. Derivatization with diazomethane was determined in the range of 83,7 to 110,6 % with relative standard deviation from 2,1 to 14,2 %. Aplication of the optimized method of the artificially contaminated soil samples in the analysis of GC/MS provided the yield of all analytes in the range of 91,7 to 132,5 % with relative standard deviation from 2,1 to 12,1 %. Validation of methods after aplication to GC/MS gave the analytes 2- -CBA; 3-CBA; 4-CBA and 2,3,4,5-CBA the limit of...