Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem
The colorectal cancer is one of the most common tumour diseases of all. If diagnosed at early stage, there is a reasonable chance for at least five-year survival. Common methods for screening and diagnosis are relatively simple and also quite successive, however (still) not perfect - for proper diag...
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2010
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ndltd-nusl.cz-oai-invenio.nusl.cz-3157492017-06-28T04:17:34Z Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem Biochip DNA analysis in the detection of colorectal cancer related mutations Adámek, Petr Vaníčková, Zdislava Kovářová, Jaroslava The colorectal cancer is one of the most common tumour diseases of all. If diagnosed at early stage, there is a reasonable chance for at least five-year survival. Common methods for screening and diagnosis are relatively simple and also quite successive, however (still) not perfect - for proper diagnosis an invasive procedure is necessary. In general there is a significant effort to make the procedures of screening and diagnosis more comfortable for patients as well (to make them) more precise - which means that there is great chance for employing methods of molecular genetics and biology including biochip methods. The purpose of this final-year thesis was to test the possibility of the optimization of this biochip method, originally developed by the manufacturer for ~ 6 ~ testing DNA extracted from faeces, for samples of genomic DNA extracted from white blood cells with the objective to detect possible mutations in four genes of the interest (K-ras, BRAF, TP53 and APC) with a strong relation to this cancer. The evaluation of samples was made on a biochip analyzer Evidence Investigator TM. In the final -year thesis is a summary of theoretical evidence in biological, clinical and also technical side of this affair. The biochip method is described together with an example of the actual procedure, presentation... 2010 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-315749 cze info:eu-repo/semantics/restrictedAccess |
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Czech |
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Dissertation |
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The colorectal cancer is one of the most common tumour diseases of all. If diagnosed at early stage, there is a reasonable chance for at least five-year survival. Common methods for screening and diagnosis are relatively simple and also quite successive, however (still) not perfect - for proper diagnosis an invasive procedure is necessary. In general there is a significant effort to make the procedures of screening and diagnosis more comfortable for patients as well (to make them) more precise - which means that there is great chance for employing methods of molecular genetics and biology including biochip methods. The purpose of this final-year thesis was to test the possibility of the optimization of this biochip method, originally developed by the manufacturer for ~ 6 ~ testing DNA extracted from faeces, for samples of genomic DNA extracted from white blood cells with the objective to detect possible mutations in four genes of the interest (K-ras, BRAF, TP53 and APC) with a strong relation to this cancer. The evaluation of samples was made on a biochip analyzer Evidence Investigator TM. In the final -year thesis is a summary of theoretical evidence in biological, clinical and also technical side of this affair. The biochip method is described together with an example of the actual procedure, presentation... |
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Vaníčková, Zdislava |
author_facet |
Vaníčková, Zdislava Adámek, Petr |
author |
Adámek, Petr |
spellingShingle |
Adámek, Petr Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
author_sort |
Adámek, Petr |
title |
Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
title_short |
Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
title_full |
Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
title_fullStr |
Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
title_full_unstemmed |
Využití biočipové analýzy DNA k detekci mutací souvisejících s kolorektálním karcinomem |
title_sort |
využití biočipové analýzy dna k detekci mutací souvisejících s kolorektálním karcinomem |
publishDate |
2010 |
url |
http://www.nusl.cz/ntk/nusl-315749 |
work_keys_str_mv |
AT adamekpetr vyuzitibiocipoveanalyzydnakdetekcimutacisouvisejicichskolorektalnimkarcinomem AT adamekpetr biochipdnaanalysisinthedetectionofcolorectalcancerrelatedmutations |
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1718474354570821632 |