Aktivace neuroglií po epileptickém statu vyvolaném intracerebroventrikulární aplikací 4-aminopyridinu u potkana.

• Main Author: Uttl, Libor
• Other Authors: Otáhal, Jakub
• Format: Dissertation
• Language: Czech
• Published: 2013
• Online Access: http://www.nusl.cz/ntk/nusl-323581

Epilepsy is a chronic disease characterized by spontaneous epileptic seizures. One percent of the world population is affected by the epilepsy. The existence of proper models is crucial for study of distinct types of epilepsy. We decided to deeply describe the model of status epilepticus (SE) induction in an adult rat by the intracerebroventricular application of 4-aminopyridine (4-AP). For description of this model we used the histology and immunohistochemistry methods, the western blot analysis focused on the microglial activation markers, and the series of behavioral tests to reveal the functional influence of SE in chronical experiment (8 months). We described the ability of the 4-AP to induce SE in this model. After SE, neuron degeneration and microglial activation appeared. The areas of degenerated neurons strongly corresponded to the regions with activated microglias. By the western blotting we demonstrated the oxidative stress by an increase of 3-nitrotyrosine already 2 hours after SE. Microglial activation detected by the immunohistochemistry correlated to the observed increase in IL-1β and CD68 production after SE. Considering these results we verified the functional influence of the SE in chronical experiment. We did not observed any significant changes of cognitive and motoric functions...