| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Zuzana Bártová Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma thesis: Quantum-Dots particles as enhancers for chemiluminescence determinations in SIA system Quantum-Dots were tested as enhancers of chemiluminescence (CL) emission using sequential injection analysis (SIA). A CL reaction used for this testing was oxidation of luminol (5-aminophthalylhydrazide) by hydrogen peroxide in alkaline medium, QDs represented enhancer of CL signal. The following concentrations of the solutions were tested: hydrogen peroxide in the range 1.10-1 - 1.10-7 mol.l-1 , without QDs and with QDs having maximum emission at 520, 575, 603 a 636 nm and dilution of QDs in following levels 10 µl/10 ml (0.1% solution), 200 µl/10 ml (2% solution) and 200 µl/1 ml (20% solution). The CL emission was detected in two ways. The CL cell, which was constructed at the Department of Analytical Chemistry, FaF UK in HK, and photomultiplier Hamamatsu (Japan) as a detector, enabled monitoring of the CL emission including its time profile. The photomultiplier of the fluorimetric detector Schoeffel (Germany) with spiral flow cell were used for flow measurements in the SIA system. The CL emission was evaluated by...
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