| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Student: Kateřina Dittrichová Supervisor: RNDr. Milan Mokrý, CSc. Consultant: Ing. Lubomír Galla Title of master's thesis: Evaluation of selected pharmaceuticals by HPLC with spectrophotometric detection A liquid-chromatographic method was developed for the simultaneous determination of dexpanthenol, phenoxyethanol, and preservatives (methyl-, ethyl-, propyl-, isobutyl-, and butylparaben). Separation of these compounds was performed on column Discovery C18 (5 µm, 150 mm × 4.6 mm I.D.) by isocratic elution with a potassium dihydrogen phosphate buffer (0.01 mol l-1, pH adjusted to 2.5 with a phosphoric acid 85 %) and an acetonitrile (67:33, v/v) at the flow rate of 1.00 ml min-1. The injection volume of 5 µl was used. The diode array detector operated at two wavelengths (210 nm for dexpanthenol and 254 nm for phenoxyethanol and preservatives). Three basic system suitability parameters were evaluated: the number of the theoretical plates of all compounds was greater than 6000 units, determined symmetry factors of all peaks were in interval from 1.1 to 1.3, and calculated values of resolution were greater than 1.5. Accuracy, precision, linearity, specificity, and selectivity tests...
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