Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Student: Kateřina Dittrichová Supervisor: RNDr. Milan Mokrý, CSc. Consultant: Ing. Lubomír Galla Title of master's thesis: Evaluation of selected pharmaceuticals by HPLC w...

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Main Author: Dittrichová, Kateřina
Other Authors: Mokrý, Milan
Format: Dissertation
Language:English
Published: 2013
Online Access:http://www.nusl.cz/ntk/nusl-329237
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spelling ndltd-nusl.cz-oai-invenio.nusl.cz-3292372021-03-29T05:12:33Z Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí Evaluation of selected pharmaceuticals by HPLC with spectrophotometric detection Dittrichová, Kateřina Mokrý, Milan Kastner, Petr Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Student: Kateřina Dittrichová Supervisor: RNDr. Milan Mokrý, CSc. Consultant: Ing. Lubomír Galla Title of master's thesis: Evaluation of selected pharmaceuticals by HPLC with spectrophotometric detection A liquid-chromatographic method was developed for the simultaneous determination of dexpanthenol, phenoxyethanol, and preservatives (methyl-, ethyl-, propyl-, isobutyl-, and butylparaben). Separation of these compounds was performed on column Discovery C18 (5 µm, 150 mm × 4.6 mm I.D.) by isocratic elution with a potassium dihydrogen phosphate buffer (0.01 mol l-1, pH adjusted to 2.5 with a phosphoric acid 85 %) and an acetonitrile (67:33, v/v) at the flow rate of 1.00 ml min-1. The injection volume of 5 µl was used. The diode array detector operated at two wavelengths (210 nm for dexpanthenol and 254 nm for phenoxyethanol and preservatives). Three basic system suitability parameters were evaluated: the number of the theoretical plates of all compounds was greater than 6000 units, determined symmetry factors of all peaks were in interval from 1.1 to 1.3, and calculated values of resolution were greater than 1.5. Accuracy, precision, linearity, specificity, and selectivity tests... 2013 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-329237 eng info:eu-repo/semantics/restrictedAccess
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language English
format Dissertation
sources NDLTD
description Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Student: Kateřina Dittrichová Supervisor: RNDr. Milan Mokrý, CSc. Consultant: Ing. Lubomír Galla Title of master's thesis: Evaluation of selected pharmaceuticals by HPLC with spectrophotometric detection A liquid-chromatographic method was developed for the simultaneous determination of dexpanthenol, phenoxyethanol, and preservatives (methyl-, ethyl-, propyl-, isobutyl-, and butylparaben). Separation of these compounds was performed on column Discovery C18 (5 µm, 150 mm × 4.6 mm I.D.) by isocratic elution with a potassium dihydrogen phosphate buffer (0.01 mol l-1, pH adjusted to 2.5 with a phosphoric acid 85 %) and an acetonitrile (67:33, v/v) at the flow rate of 1.00 ml min-1. The injection volume of 5 µl was used. The diode array detector operated at two wavelengths (210 nm for dexpanthenol and 254 nm for phenoxyethanol and preservatives). Three basic system suitability parameters were evaluated: the number of the theoretical plates of all compounds was greater than 6000 units, determined symmetry factors of all peaks were in interval from 1.1 to 1.3, and calculated values of resolution were greater than 1.5. Accuracy, precision, linearity, specificity, and selectivity tests...
author2 Mokrý, Milan
author_facet Mokrý, Milan
Dittrichová, Kateřina
author Dittrichová, Kateřina
spellingShingle Dittrichová, Kateřina
Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
author_sort Dittrichová, Kateřina
title Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
title_short Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
title_full Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
title_fullStr Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
title_full_unstemmed Hodnocení vybraných léčiv pomocí HPLC se spektrometrickou detekcí
title_sort hodnocení vybraných léčiv pomocí hplc se spektrometrickou detekcí
publishDate 2013
url http://www.nusl.cz/ntk/nusl-329237
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AT dittrichovakaterina evaluationofselectedpharmaceuticalsbyhplcwithspectrophotometricdetection
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