Využití systému přepínání kolon v HPLC pro zakoncentrování a stanovení insekticidů z vodné matrice

• Main Author: Naibrtová, Linda
• Other Authors: Šatínský, Dalibor
• Format: Dissertation
• Language: Czech
• Published: 2014
• Online Access: http://www.nusl.cz/ntk/nusl-333062

This diploma thesis deals with development and optimization of conditions of HPLC method for a suitable pre-concentration and determination of two pesticides - fenoxycarb and permethrin in surface waters. The on-line SPE HPLC with column switching system was used for method development and determination. To validate the optimal conditions there was chosen analytical column Supelco Ascentis Express Phenyl - Hexyl (100 x 4.6 mm), particle size of 2.7 microns. Detection was carried out at wavelength 225 nm and column temperature was set on 45 řC using gradient elution with a mobile phase of acetonitrile - water at a flow rate of 1,0 ml/min. Prior to analysis, the sample was pre-concentrated using an extraction precolumn Ascentis Express C-18 (5 x 4.6 mm), particle size of 2.7 micron with washing mobile phase of 30% acetonitrile in water at a flow rate of 0.5 ml/min. The injected sample volume was optimized to 1500 μl of water sample. An original method, which allows the determination of two selected pesticides in real matrix surface waters without difficult sample pre-treatment was developed.