| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Student: Martina Hynková Supervisor: RNDr. Milan Mokrý, CSc. Diploma thesis: HPLC evaluation of selected pharmaceuticals VIII RP-HPLC method for simultaneous analysis of Paracetamol, Ascorbic Acid and Phenylephrine in pharmaceutical formulation (powder for oral solution) was developed. In this formulation, the amount of Paracetamol is sixtimes higher then the amount of Phenylephrine. Macherey-Nagel column NUCLEOSIL C18 (150 mm x 4 mm id, 5 µm particle size) was used as a sorbent. Isocratic elution with mobile phase composed of 0,001M monopotassium phosphate puffer and organic modifier methanol (80 : 20, V/V; pH 3,00 - adjusted with 50% phosphoric acid) at a flow rate of 0,7 ml.min-1 and 25 řC in less then 14 minutes. UV detection at the wavelenght 225 nm. Sample volume 10 µl. The influence of variations in mobile phase parameters (pH, content of organic modifier and puffer concentration) was observed. With allopurinol as an IS, calibration was performed to enable quantification of drugs in pharmaceutical formulation.
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