Identifikace bakterií z pozitivních hemokultur pomocí metody hmotnostní spektrometrie
CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and medical Sciences Candidate: Bc. Aneta Jahnová Supervisor: MUDr. Pavla Paterová, Dept. of Clinical Microbiology FN Hradec Králové Consultant: primářka MUDr. Alena Ševčíková, Dept. of Clinical Microbiology...
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| Format: | Dissertation |
| Language: | Czech |
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2014
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| Online Access: | http://www.nusl.cz/ntk/nusl-336629 |
| Summary: | CHARLES UNIVERSITY IN PRAGUE Faculty of Pharmacy in Hradec Králové Department of Biological and medical Sciences Candidate: Bc. Aneta Jahnová Supervisor: MUDr. Pavla Paterová, Dept. of Clinical Microbiology FN Hradec Králové Consultant: primářka MUDr. Alena Ševčíková, Dept. of Clinical Microbiology FN Brno Title of diploma thesis: Identification of bacteria from positive blood cultures by mass spectrometry. Background: During processing positive blood culture at the Department of Clinical Microbiology, University Hospital Brno MALDI-TOF MS is used to for rapid and reliable identification cultured bacteria growing pure culture after five to six hours on solid culture media. The aim of this study was evaluated and compared methods for direct identification of the etiological agents from positive blood cultures using a kit SepsiTyperTM and test tubes with gel, which should decrease the time to obtain the result. Methods: In this study were tested 50 positive BacT/ALERT blood cultures. Preparation of microbial samples was carried out parallel using a kit SepsiTyperTM and collection test tubes with gel. In both methods was performed extraction with ethanol/formic acid and analyses on a Microflex LT system. Results: From fifty testing blood cultures were correctly identified by kit SepsiTyper 24 samples... |
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