Studie interakce mezi lidským AQP5 a PIP
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences University of Gothenburg Faculty of Science Department of Chemistry and Molecular Biology Candidate: Veronika Nešverová Supervisor: Vladimír Wsól Title of diploma thesis: Studies of the interaction...
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ndltd-nusl.cz-oai-invenio.nusl.cz-3373952017-06-28T04:19:31Z Studie interakce mezi lidským AQP5 a PIP Studies of the interaction between human AQP5 and PIP Nešverová, Veronika Wsól, Vladimír Zemanová, Lucie Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences University of Gothenburg Faculty of Science Department of Chemistry and Molecular Biology Candidate: Veronika Nešverová Supervisor: Vladimír Wsól Title of diploma thesis: Studies of the interaction between human AQP5 and PIP Aquaporin 5 is a membrane canal which maintains water balance mainly in lacrimal and salivary glands. A defect in this protein's trafficking from cytoplasm to cytoplasmic membrane is thought to play a role in development of Sjögren's syndrome - a chronic autoimmune disease. Prolactin-inducible protein is a cytoplasmic protein which was found to be contributing to this disease's pathogenesis. This project was aimed to study the proposed interaction between aquaporin 5 and prolactin-inducible protein. Both recombinant proteins were overexpressed in the yeast Pichia pastoris and purified using low-pressure column chromatography on an ÄKTA purification system. Glycosylation of prolactin-inducible protein was confirmed using specific glycoprotein staining. Two coelution tries were run on a HisTrap column. The fractions eluted from the second try were analysed by mass spectrometry. However results did not confirm any interaction. Further investigation is needed for the complete... 2014 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-337395 eng info:eu-repo/semantics/restrictedAccess |
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Dissertation |
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Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences University of Gothenburg Faculty of Science Department of Chemistry and Molecular Biology Candidate: Veronika Nešverová Supervisor: Vladimír Wsól Title of diploma thesis: Studies of the interaction between human AQP5 and PIP Aquaporin 5 is a membrane canal which maintains water balance mainly in lacrimal and salivary glands. A defect in this protein's trafficking from cytoplasm to cytoplasmic membrane is thought to play a role in development of Sjögren's syndrome - a chronic autoimmune disease. Prolactin-inducible protein is a cytoplasmic protein which was found to be contributing to this disease's pathogenesis. This project was aimed to study the proposed interaction between aquaporin 5 and prolactin-inducible protein. Both recombinant proteins were overexpressed in the yeast Pichia pastoris and purified using low-pressure column chromatography on an ÄKTA purification system. Glycosylation of prolactin-inducible protein was confirmed using specific glycoprotein staining. Two coelution tries were run on a HisTrap column. The fractions eluted from the second try were analysed by mass spectrometry. However results did not confirm any interaction. Further investigation is needed for the complete... |
author2 |
Wsól, Vladimír |
author_facet |
Wsól, Vladimír Nešverová, Veronika |
author |
Nešverová, Veronika |
spellingShingle |
Nešverová, Veronika Studie interakce mezi lidským AQP5 a PIP |
author_sort |
Nešverová, Veronika |
title |
Studie interakce mezi lidským AQP5 a PIP |
title_short |
Studie interakce mezi lidským AQP5 a PIP |
title_full |
Studie interakce mezi lidským AQP5 a PIP |
title_fullStr |
Studie interakce mezi lidským AQP5 a PIP |
title_full_unstemmed |
Studie interakce mezi lidským AQP5 a PIP |
title_sort |
studie interakce mezi lidským aqp5 a pip |
publishDate |
2014 |
url |
http://www.nusl.cz/ntk/nusl-337395 |
work_keys_str_mv |
AT nesverovaveronika studieinterakcemezilidskymaqp5apip AT nesverovaveronika studiesoftheinteractionbetweenhumanaqp5andpip |
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1718476225285980160 |