Ekologické a evoluční důsledky polyploidizace

• Main Author: Pavlíková, Zuzana
• Other Authors: Münzbergová, Zuzana
• Format: Dissertation
• Language: Czech
• Published: 2014
• Online Access: http://www.nusl.cz/ntk/nusl-337626

Artificially synthesized neopolyploids are commonly used to distinguish the direct consequences of polyploidization for plants from those that were formed in polyploid line during subsequent evolution. Colchicine is usually used for induction of somatic polyploids. In this work I tested the possibility of making neopolyploids by using colchicine in three selected plant species. Succcess of neotetraploids was 9,3 % of the species Vicia cracca, 31,6 % of the species Centaurea phrygia and 33,3 % of the species Pimpinella saxifraga when using a 0,2% solution of colchicine and the effect of 12 hours. When extending the exposure time to 18 hours at the same concentration of colchicine in Vicia cracca the success of polyploidization was 100 % but mortality of individuals threated with colchicine was nearly 98 % (when exposed 12 hours it was only 43 %). Vicia cracca was grown to the second generation of neopolyploid plants, but part of the individuals was aneuploid and they were not phenotypically different from the natural tetraploids. Natural diploids, tetraploids and neotetraploids were compared in the size of stomata, rate of germination and relative growth rate. The size of stomata of diploids was significantly different from tetraploids and neotetraploids so it is possible to say that...