Studium regulace regenerace jater za využití metod molekulární genetiky.

• Main Author: Rychtrmoc, David
• Other Authors: Červinková, Zuzana
• Format: Doctoral Thesis
• Language: Czech
• Published: 2014
• Online Access: http://www.nusl.cz/ntk/nusl-338485

Regulation of liver regeneration as assessed by means of molecular genetics Liver regeneration is a noteworthy phenomenon of a vigorous tissue recovery following a wide variety of damaging events. Regenerative ability of the liver is unique among the mammalian visceral organs and plays its role in pathophysiology of heterogeneous hepatic diseases. The recovery process occurs in three phases of which the termination remains the least elucidated. Even the questions of regeneration termination timing and of completeness of organ build-up are still controversial despite decades of studies. The aim of the present thesis was to analyse gene expression data pertinent to late phase of liver regeneration.We evaluated the course of spontaneous recovery in rats subjected to 2/3 partial hepatectomy. Because of lack of generally agreed parameter of hepatic regeneration completeness we proposed a proprietary indicator denoted as %LReg, based on organ and whole body weight assessments performed throughout the study. Gene expression analysis was first performed using DNA microarrays and consequently by real-time RT-PCR. Whole genome scale transcriptional analysis yielded vast amount of data covering gene expression dynamics of fourteen post-surgery days after 2/3 partial hepatectomy in adult male rats. This intervention in...