Studium fázového chování vybraných směsi lipidů
Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: Mgr. Jarmila Zbytovská, Dr. rer. nat. Student: Klára Černochová Title of Thesis: Study on phase behaviour of selected lipid mixtures Ceramides, cholesterol and fatty acids are the...
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ndltd-nusl.cz-oai-invenio.nusl.cz-3440732017-06-28T04:20:27Z Studium fázového chování vybraných směsi lipidů Study on phase behaviour of selected lipid mixtures Černochová, Klára Zbytovská, Jarmila Matějka, Pavel Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: Mgr. Jarmila Zbytovská, Dr. rer. nat. Student: Klára Černochová Title of Thesis: Study on phase behaviour of selected lipid mixtures Ceramides, cholesterol and fatty acids are the main components of the stratum corneum (SC) intercellular lipid matrix. These lipids play the major role in maintaining the skin barrier function. The goal of this work is to study the SC lipid mixtures on the molecular level, characterization of their miscibility and their temperature phase behavior. For this purpose, lipid model systems imitating natural structure of the SC intercellular lipid matrix were prepared. The membranes contained one or more ceramides (CerAP, CerNP, CerNS), cholesterol, stearic acid and cholesterol sulfate. The mixtures were studied by infrared spectroscopy using a heated ATR-crystal. From the measured data, IR spectra and temperature dependency of the position of the particular IR-modes were evaluated. The results showed the presence of hydrogen bindings within the ceramides. These bonds were also observable in the equimolar lipid mixture based on CerNP. Otherwise, lipid mixtures containing CerAP showed very good miscibility of the individual components. The two- and three-... 2016 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-344073 cze info:eu-repo/semantics/restrictedAccess |
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Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: Mgr. Jarmila Zbytovská, Dr. rer. nat. Student: Klára Černochová Title of Thesis: Study on phase behaviour of selected lipid mixtures Ceramides, cholesterol and fatty acids are the main components of the stratum corneum (SC) intercellular lipid matrix. These lipids play the major role in maintaining the skin barrier function. The goal of this work is to study the SC lipid mixtures on the molecular level, characterization of their miscibility and their temperature phase behavior. For this purpose, lipid model systems imitating natural structure of the SC intercellular lipid matrix were prepared. The membranes contained one or more ceramides (CerAP, CerNP, CerNS), cholesterol, stearic acid and cholesterol sulfate. The mixtures were studied by infrared spectroscopy using a heated ATR-crystal. From the measured data, IR spectra and temperature dependency of the position of the particular IR-modes were evaluated. The results showed the presence of hydrogen bindings within the ceramides. These bonds were also observable in the equimolar lipid mixture based on CerNP. Otherwise, lipid mixtures containing CerAP showed very good miscibility of the individual components. The two- and three-... |
author2 |
Zbytovská, Jarmila |
author_facet |
Zbytovská, Jarmila Černochová, Klára |
author |
Černochová, Klára |
spellingShingle |
Černochová, Klára Studium fázového chování vybraných směsi lipidů |
author_sort |
Černochová, Klára |
title |
Studium fázového chování vybraných směsi lipidů |
title_short |
Studium fázového chování vybraných směsi lipidů |
title_full |
Studium fázového chování vybraných směsi lipidů |
title_fullStr |
Studium fázového chování vybraných směsi lipidů |
title_full_unstemmed |
Studium fázového chování vybraných směsi lipidů |
title_sort |
studium fázového chování vybraných směsi lipidů |
publishDate |
2016 |
url |
http://www.nusl.cz/ntk/nusl-344073 |
work_keys_str_mv |
AT cernochovaklara studiumfazovehochovanivybranychsmesilipidu AT cernochovaklara studyonphasebehaviourofselectedlipidmixtures |
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1718477199323955200 |