Nové přístupy k přípravě scaffoldů pro tkáňové inženýrství odolných proti nespecifické adsorpci proteinů a adhezi buněk

• Main Author: Kostina, Nina
• Other Authors: Michálek, Jiří
• Format: Doctoral Thesis
• Language: English
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-347496

Non-specific protein adsorption -protein fouling- is an adverse effect occurring at the surface of most artificial materials which come into contact with biological fluids, affecting various biomedical devices and tissue engineering scaffolds. In order to address this ubiquitous problem the preparation of scaffolds resistant to protein fouling (antifouling) was proposed. The main goal of this Thesis was the development of strategies for the preparation of antifouling scaffolds for tissue engineering. The work consisted of three steps: (i) the selection of monomers leading to antifouling properties by utilizing model system based on polymer brushes; (ii) preparation of antifouling hydrogels of different properties and architecture by copolymerization with zwitterionic carboxybetaine monomers, selected from model experiment; and (iii) modification of the surface of poly( -caprolactone) (PCL) nanofibers by growing four types of polymer brushes resistant to protein fouling. The scaffolds presented in this work showed variety of unique properties, such as hydration (up to 30000%), elongation (up to 1800%), self-healing, biodegradation, availability of functional groups and complex pore structure. Importantly, all scaffolds showed significant reduction of protein fouling and non-specific cell adhesion and can be...