Koimunoprecipitace jako nástroj pro studium interakcí DHRS7 enzymu s proteiny

• Main Author: Fassmannová, Dominika
• Other Authors: Zemanová, Lucie
• Format: Dissertation
• Language: Czech
• Published: 2016
• Online Access: http://www.nusl.cz/ntk/nusl-347940

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Dominika Fassmannová Supervisor: RNDr. Lucie Zemanová, Ph.D. Title of diploma thesis: Co-immunoprecipitace as a tool for the study of protein-protein interactions of DHRS7 enzyme DHRS7 enzyme is a member of short-chain dehydrogenase/reductase superfamily. The enzyme is at least in vitro NADPH-dependent reductase of some substance bearing carbonyl group, including androstenedione or all-trans retinal. DHRS7 is close homolog of well-known 11β-hydroxysteroid dehydrogenase 1. Physiological function of DHRS7 is unknown, the level of the knowledge is quite low. Recently, information about some role of DHRS7 in diseases as prostate cancer or insulin resistance was published. A role of poorly characterized proteins as DHRS7 can be predicted based on evidence of their interaction with a protein with revealed function, because proteins inside cells do not usually work alone, they are part of a huge protein complex known as interactome. Knowledge of such protein-protein interactions help us to understand the function and regulation of the protein inside a cell or organism. The aim of the study is initial investigation of proteins interacting with DHRS7 by co-immunoprecipitation, a basic method for...