| Summary: | Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Control Candidate: Štěpán Skopový Consultant: RNDr. Milan Mokrý, CSc. Title of Thesis: HPLC evaluation of some drugs XI. The main focus of this diploma thesis was to find suitable chromatografic conditions for HPLC analysis of Fenofibrate and Ketorolac. Aproximate chromatographic conditions for analysis of Fenofibrate were suggested based on review of previous research projects concerning HPLC analysis of this drug. As for the stacionary phase, a C18 column (250 x 4.6 mm, 5µm particle size) was considered to be the most suitable. Suggested mobile phase is to be formed by mixture of acetonitrile and phosfate buffer (pH aproximetly 3,6) in the ratio 50: 50 or 60:40. Detection could be carried out by an UV-VIS detector at 286 nm. For the analysis of ketorolac it was used Sigma-Aldrich's Discovery HS C18 HPLC column (150x4.6 mm, 5 μm). The mobile phase consisted of acetonitrile and 0,01 mol/l potassium dihydrogen phosphate buffer (pH 3.25) in the ratio 40:60 (v/v). Isocratic elution at a flow rate of 1 ml/minute was set at temperature 25řC and the maximum pressure was 20 Mpa. Thiaprofenic acid was used as internal standard and the detection was carried out by a PDA detector (model SPD-M20...
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