| Summary: | Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Martina Procházková Supervisor: Doc. PharmDr. Hana Sklenářová, Ph.D. Title of diploma thesis: Application of Raman spectrometry for analysis of selected mixtures The identification and determination of methanol and ethanol using Raman spectroscopy was tested. For such tests two types of calibration standards were prepared. The volumes of analytes used were adjusted to get the final concentration of the alcohols corresponding to 40 volume %. In the first type of calibration varied amounts of methanol and ethanol were applied, in the second different amount of methanol was used. Measurement was carried out using portable dispersive Raman spectrometer with laser with an excitation wavelength of 785 nm. The effect of the measurement conditions to linearity of the calibration obtained in the evaluation of the signal intensity and the area of the intense band in the spectrum were tested in detail. Measurement conditions included the integration time (1000 ms, 2500 ms, 5000 ms and 7500 ms), the laser intensity (100 and 75%) and temperature (laboratory and 4-8 řC). Calibration standards and samples were measured in glass vials. To identify the analytes whole Raman spectrum was compared. To compare the...
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