| Summary: | Charles University Faculty of Pharmacy in Hradec Králové Department of pharmaceutical chemistry and drug analysis Candidate: Libuše Klepalová Supervisor: doc. PharmDr. Petra Kovaříková, Ph.D. Title of the Master's Thesis: Analytical and bioanalytical study of selected drugs using UHPLC Abstract High-performance liquid chromatography coupled with mass spectrometry plays a crucial role in drug analysis from biological materials, mainly due to its sensitivity, specificity and ability to provide structural information on the analytes. Multiple myeloma is a serious hematologic disease, accounting for about 1 % of all oncological diseases. New drugs from the group of proteasome inhibitors namely, carfilzomib and bortezomib represent a great promises for patients with relapsing multiple myeloma or in the case of the resistant to other anticancer drugs. The aim of this work was to develop pilot chromatographic conditions for HPLC-MS analysis of carfilzomib and bortezomib and evaluate different extraction of these drugs from plasma. Separation was carried out on an Ascentis column C18 10 x 3 mm, 3 µm, (Supelco, Germany). Electrospray ionization with ion trap mass analyzer were used in both cases. The mobile phase consisting of water and acetonitrile (20:80) was the most appropriate for carfilzomib. The flow rate of...
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