Studium kožních onemocnění pomocí monovrstevných lipidových modelů

• Main Author: Mihalová, Veronika
• Other Authors: Školová, Barbora
• Format: Dissertation
• Language: Czech
• Published: 2017
• Online Access: http://www.nusl.cz/ntk/nusl-356258

Charles University, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Technology Consultant: PharmDr. Barbora Školová, Ph.D. Student: Veronika Mihalová Title of Thesis: Study of skin diseases using monolayer lipid models Skin is an organ that protects the body against external influences and excessive water loss. The proper skin barrier is localized in the outermost layer of the epidermis, the stratum corneum (SC). The function of this barrier depends on the composition and organization of the extracellular space of SC. It consists mainly of ceramides (CER), free fatty acids and cholesterol in approximately equimolar ratio. Imbalances in the lipid composition are associated with skin diseases such as atopic dermatitis. Ceramidase is an enzyme which cleaves CER or glucosylated ceramides (precursor of CER) to lysosphingolipid (LysoSph) and fatty acid. Abundance or deficit of this enzyme causes dysfunction of skin barrier and the manifestation of skin diseases, e.g. Farber's disease. The aim of this work was to study the monolayer lipid models whose compositions reflect the abundance of ceramidase in the skin. Lipid models were studied by Langmuir monolayers at the air-liquid interface and at the solid surface, including Brewster angle microscopy and atomic force microscopy. With...