Studium metabolismu polyaminů v buněčném dělení a jejich úloha ve fyziologických pochodech rostlin
3. AIMS To study the role of PA biosynthesis,oxidation and conjugation in maintenanceof free intracellular PA levelsduring the growth and cell cycle of tobacco BY-2 cell culture (Nicotiana tabacum L.cv. Bright Yellow-2). Partial aims: o To describe alterations in the levels of free PA and PA conjuga...
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| Format: | Doctoral Thesis |
| Language: | Czech |
| Published: |
2009
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| Online Access: | http://www.nusl.cz/ntk/nusl-370569 |
| Summary: | 3. AIMS To study the role of PA biosynthesis,oxidation and conjugation in maintenanceof free intracellular PA levelsduring the growth and cell cycle of tobacco BY-2 cell culture (Nicotiana tabacum L.cv. Bright Yellow-2). Partial aims: o To describe alterations in the levels of free PA and PA conjugated with HCA during the growth and cell cycle oftobaccoBY-2 cell culture. o To charaďerjzechangesin the activities of PA bioqynthetic en4/mes(ADC, ODC and SAMDC) during the growth and cell cycle oftobacco BY-2 cell culture. o To detenrrinatechangesin the activiý of PA degradation en4fme (DAO) duringthegrowthandcell cycle of tobacco BY-2 cell culture. Following (next)aims: o To evaluatealterationsin the levels of intracellular free and conjugatedPA in tobacco BY-2 cell culture under Cd2*- inducedoxidativestress. o To studythemechanismsof PA homeostasis(biosynthesis, degradation and conjugation) during the diurnal cycle oftobacco plant(Nicotiana tabacumL. cv. Wisconsin 38). o To evaluate the role of excreted PA in PA homeostasis dwing the growth cycle of tobacco BY-2 and alfalfa (MedicagosativaL.) cell suspensionculture. o To comparechangesin the PA metabolismduring somatic and zygotic embryodevelopmentof Norway spruce(Picea abiesL. (Kars|). 7 |
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