Summary: | Charles University, Faculty of Pharmacy in Hradec Kralove Department of Analytical Chemistry Candidate: Mgr. Eva Kasalová Supervisor: Prof. RNDr. Petr Solich, CSc. Supervisor specialist: Assoc. Prof. RNDr. Dagmar Solichová, Ph.D. Title of Doctoral Thesis: Modern separation techniques for the analysis of biological material in clinical research This doctoral thesis deals with development and validation of a chromatographic determination of liposoluble vitamins and with optimization of biological materials preparation. Clinical monitoring of these vitamins, especially vitamin D in different biological materials is one of the most discuss theme in the last decade. In the theoretical part of this dissertation the target analytes - vitamins A, E and D with their biological activity are characterized - as well as the biological material used in the Research Laboratory, especially a detailed description of the composition of breast milk, its irreplaceable role in neonatal nutrition and description of breast milk processing in Milk Bank. This part is followed by a comprehensive chapter dealing with the treatment of the biological matrix before analysis. The most commonly used sample pretreatment techniques such as extraction methods are described here, with a great description of new trends in these...
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