Screening systémových amyloidóz v materiálu endoskopických biopsií.
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in...
Main Author: | |
---|---|
Other Authors: | |
Format: | Dissertation |
Language: | Czech |
Published: |
2018
|
Online Access: | http://www.nusl.cz/ntk/nusl-380214 |
id |
ndltd-nusl.cz-oai-invenio.nusl.cz-380214 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-nusl.cz-oai-invenio.nusl.cz-3802142018-11-16T04:18:23Z Screening systémových amyloidóz v materiálu endoskopických biopsií. Screening for systemic amyloidoses in endoscopic biopsy samples. Květoň, Martin Nachtigal, Petr Němečková, Ivana Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique.... 2018 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-380214 cze info:eu-repo/semantics/restrictedAccess |
collection |
NDLTD |
language |
Czech |
format |
Dissertation |
sources |
NDLTD |
description |
Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Diploma thesis Candidate: Bc. Martin Květoň Branch of study: Specialist in Laboratory Methods Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D. Title: Screening for systemic amyloidoses in endoscopic biopsy samples BACKGROUND Systemic amyloidoses constitute a complex and heterogeneous group of serious diseases that result from protein misfolding and whose common feature is the deposition of pathological protein substances (amyloid) in the extracellular space of tissues. Diagnostics of amyloidoses is based on histopathological examination of tissue samples and direct demonstration of the presence of amyloid deposits. The overall purpose of this diploma thesis was to create, apply and subsequently evaluate a screening program focused on systemic amyloidoses. METHODS In the first part of the experimental study, available staining techniques used for the detection of amyloid deposits in the tissue were compared. The second part of the experimental study consisted of performing screening examinations on endoscopic biopsy samples of the gastrointestinal tract. RESULTS Based on the results of comparison, a method employing the Sirius red F3B dye was chosen as a suitable screening technique.... |
author2 |
Nachtigal, Petr |
author_facet |
Nachtigal, Petr Květoň, Martin |
author |
Květoň, Martin |
spellingShingle |
Květoň, Martin Screening systémových amyloidóz v materiálu endoskopických biopsií. |
author_sort |
Květoň, Martin |
title |
Screening systémových amyloidóz v materiálu endoskopických biopsií. |
title_short |
Screening systémových amyloidóz v materiálu endoskopických biopsií. |
title_full |
Screening systémových amyloidóz v materiálu endoskopických biopsií. |
title_fullStr |
Screening systémových amyloidóz v materiálu endoskopických biopsií. |
title_full_unstemmed |
Screening systémových amyloidóz v materiálu endoskopických biopsií. |
title_sort |
screening systémových amyloidóz v materiálu endoskopických biopsií. |
publishDate |
2018 |
url |
http://www.nusl.cz/ntk/nusl-380214 |
work_keys_str_mv |
AT kvetonmartin screeningsystemovychamyloidozvmaterialuendoskopickychbiopsii AT kvetonmartin screeningforsystemicamyloidosesinendoscopicbiopsysamples |
_version_ |
1718791745948352512 |