| Summary: | Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Candidate: Eva Hanková Supervisor: RNDr. Milan Mokrý, CSc. Title of Diploma Thesis: Analytical evaluation of drugs using HPLC II. Abstract This diploma thesis dealt with analytical evaluation of tiaprofenic acid using high performance liquid chromatography. To determine tiaprofenic acid, a LiChroCART® 250-4 LiChrospher® 100 RP-18 (5 μm) column and a mobile phase consisting of acetonitrile and aqueous KH2PO4 buffer 0.01 mol/l in a ratio of 40 : 60 (v/v) were used. The pH of the buffer was adjusted to 3.0 with phosphoric acid; the flow rate was set to 1 ml/min and the temperature at 22 řC. Naproxen was chosen as the internal standard, and a 263 nm wavelength was used for detection. Since the tiaprofenic acid is a chiral substance present in the form of a racemate, the selection of chromatographic conditions for the separation of its enantiomers followed. Two chiral columns in both normal and reverse mode were tested. The cellulose-based Chiralcel OD-R 250 x 4.6 mm (10 μm) column was chosen as the most suitable, using a mobile phase of n-hexane : propan-2-ol : acetic acid in a ratio of 94 : 6 : 0,1 (v/v /v) at a flow rate of 0.5 ml/min and a temperature of 22 ř C. Enantiomers were eluted at...
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