Analytické hodnocení léčiv s využitím HPLC II.
Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Candidate: Eva Hanková Supervisor: RNDr. Milan Mokrý, CSc. Title of Diploma Thesis: Analytical evaluation of drugs using HPLC II. Abstract This diploma thesis dealt with analytical evalu...
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Online Access: | http://www.nusl.cz/ntk/nusl-380429 |
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ndltd-nusl.cz-oai-invenio.nusl.cz-3804292018-11-16T04:18:23Z Analytické hodnocení léčiv s využitím HPLC II. Analytical evaluation of drugs using HPLC II. Hanková, Eva Mokrý, Milan Nobilis, Milan Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Candidate: Eva Hanková Supervisor: RNDr. Milan Mokrý, CSc. Title of Diploma Thesis: Analytical evaluation of drugs using HPLC II. Abstract This diploma thesis dealt with analytical evaluation of tiaprofenic acid using high performance liquid chromatography. To determine tiaprofenic acid, a LiChroCART® 250-4 LiChrospher® 100 RP-18 (5 μm) column and a mobile phase consisting of acetonitrile and aqueous KH2PO4 buffer 0.01 mol/l in a ratio of 40 : 60 (v/v) were used. The pH of the buffer was adjusted to 3.0 with phosphoric acid; the flow rate was set to 1 ml/min and the temperature at 22 řC. Naproxen was chosen as the internal standard, and a 263 nm wavelength was used for detection. Since the tiaprofenic acid is a chiral substance present in the form of a racemate, the selection of chromatographic conditions for the separation of its enantiomers followed. Two chiral columns in both normal and reverse mode were tested. The cellulose-based Chiralcel OD-R 250 x 4.6 mm (10 μm) column was chosen as the most suitable, using a mobile phase of n-hexane : propan-2-ol : acetic acid in a ratio of 94 : 6 : 0,1 (v/v /v) at a flow rate of 0.5 ml/min and a temperature of 22 ř C. Enantiomers were eluted at... 2018 info:eu-repo/semantics/masterThesis http://www.nusl.cz/ntk/nusl-380429 cze info:eu-repo/semantics/restrictedAccess |
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Dissertation |
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Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Drug Analysis Candidate: Eva Hanková Supervisor: RNDr. Milan Mokrý, CSc. Title of Diploma Thesis: Analytical evaluation of drugs using HPLC II. Abstract This diploma thesis dealt with analytical evaluation of tiaprofenic acid using high performance liquid chromatography. To determine tiaprofenic acid, a LiChroCART® 250-4 LiChrospher® 100 RP-18 (5 μm) column and a mobile phase consisting of acetonitrile and aqueous KH2PO4 buffer 0.01 mol/l in a ratio of 40 : 60 (v/v) were used. The pH of the buffer was adjusted to 3.0 with phosphoric acid; the flow rate was set to 1 ml/min and the temperature at 22 řC. Naproxen was chosen as the internal standard, and a 263 nm wavelength was used for detection. Since the tiaprofenic acid is a chiral substance present in the form of a racemate, the selection of chromatographic conditions for the separation of its enantiomers followed. Two chiral columns in both normal and reverse mode were tested. The cellulose-based Chiralcel OD-R 250 x 4.6 mm (10 μm) column was chosen as the most suitable, using a mobile phase of n-hexane : propan-2-ol : acetic acid in a ratio of 94 : 6 : 0,1 (v/v /v) at a flow rate of 0.5 ml/min and a temperature of 22 ř C. Enantiomers were eluted at... |
author2 |
Mokrý, Milan |
author_facet |
Mokrý, Milan Hanková, Eva |
author |
Hanková, Eva |
spellingShingle |
Hanková, Eva Analytické hodnocení léčiv s využitím HPLC II. |
author_sort |
Hanková, Eva |
title |
Analytické hodnocení léčiv s využitím HPLC II. |
title_short |
Analytické hodnocení léčiv s využitím HPLC II. |
title_full |
Analytické hodnocení léčiv s využitím HPLC II. |
title_fullStr |
Analytické hodnocení léčiv s využitím HPLC II. |
title_full_unstemmed |
Analytické hodnocení léčiv s využitím HPLC II. |
title_sort |
analytické hodnocení léčiv s využitím hplc ii. |
publishDate |
2018 |
url |
http://www.nusl.cz/ntk/nusl-380429 |
work_keys_str_mv |
AT hankovaeva analytickehodnocenilecivsvyuzitimhplcii AT hankovaeva analyticalevaluationofdrugsusinghplcii |
_version_ |
1718791757514145792 |