| Summary: | Charles University, Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Niké Hazuková Supervisor: Assoc. Prof. RNDr. Lenka Kujovská Krčmová, Ph.D. Title of diploma thesis: High performance liquid chromatogramy determination of selected amino acids and agamtine in biological material Keywords: agmatine, L-arginine, L-citruline, L-ornitine, UHPLC, chronic wounds This thesis follows up the metod development for the determination of agmatine, L- arginine, L-citrulline, L-ornithine in chronic wounds using ultra-high performance liquid chromatography. The aim of this thesis was to optimize suitable chromatographic conditions for the already mentioned analytes with possibility to use this method in clinical research and practice. Four chromatographic columns were tested. Finally the Meteoric Core C18 BIO the dimensions 100 x 4.6 mm, 16 nm, 2.7 μm (YMC, Germany) was chosen, using these conditions: the mobile phase (MF) was 100% 10 mM AMFO (pH 2.5), the temperature was set at 25 řC, the MF flow rate was 0.25 mL / min, and the injection volume was 2 μL. After optimization of the separation conditions, the method was applied to biological material (exudate from chronic wounds), samples were modified by selected extraction procedures as ultrafiltration and protein precipitation....
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