Možnosti kapilární elektroforézy pro separaci boswellových kyselin

• Main Author: Poušková, Jitka
• Other Authors: Jáč, Pavel
• Format: Dissertation
• Language: Czech
• Published: 2019
• Online Access: http://www.nusl.cz/ntk/nusl-397417

Charles University Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Bc. Jitka Poušková Supervisor: PharmDr. Pavel Jáč, Ph.D. Title of the diploma thesis: The capabilities of capillary electrophoresis in the separation of boswellic acids The main aim of this work was testing of capabilities of capillary electrophoresis for the separation of boswellic acids in Boswellia serrata extract as well as in a mixture of standards. The effect of five different buffers and some additive (such as cyclodextrins, organic modifiers or surfactants) in the background electrolyte was tested. The most suitable components of the background electrolyte, were examined at different concentrations to achieve complete separation of boswellic acids. First experiments were carried out in a fused silica capillary with 50 μm internal diameter, 48.5 cm total length and 40 cm effective length, followed by the separations in a longer capillary with 83.5 cm total length and 75 cm effective length. The separation voltage was +30 kV. The UV detection was at 200 nm, 250 nm and 280 nm. The best results were achieved with the background electrolyte composed of 80 mM phosphate buffer at pH 7.0 with 10 % (v/v) of methanol and 80 mg/ml of 2-hydroxypropyl-β- cyclodextrin. With this electrolyte, Boswellia...