Modulace protizánětlivé odpovědi sledované využitím mikroglialní buněčné linie SIM-A9

• Main Author: Tumpachová, Jitka
• Other Authors: Macháček, Miloslav
• Format: Dissertation
• Language: Czech
• Published: 2021
• Online Access: http://www.nusl.cz/ntk/nusl-446205

Charles University Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jitka Tumpachová Supervisor: RNDr. Miloslav Macháček, Ph.D. Consultant: pplk. doc. MUDr. Jaroslav Pejchal, Ph.D. et Ph.D. Title of diploma thesis: Modulation of anti-inflammatory response in microglial cell line SIM-A9 In the theoretical section, the thesis is focused on the characteristics, significance, and contribution of neuroinflammation in the origin and development of neurodegenerative diseases. The possibilities of their current treatment are discussed. The experimental part is devoted to evaluating the cytotoxicity and anti-inflammatory response of newly synthesized derivatives of naphthoquinones (K1502, K1503 and K1504) and chromones (5-EM-13, 5-EM-26 and 5-EM-36). Cytotoxicity was measured in the mouse microglial line SIM-A9. In addition, the neuroblastoma line SH-SY5Y was used comparatively in this part. The half-maximal inhibitory concentration (IC50) and maximum tolerated concentration (MTC) were determined on both cell lines using MTT colorimetric methods. The safety of MTC was subsequently verified by microcapillary flow cytometry in the SIM-A9 cells after lipopolysaccharide (LPS) or interferon-γ (IFN-γ) stimulation. The anti-inflammatory effects of the studied substances were...