Separace acikloviru a jeho degradačního produktu

• Main Author: Nodžák, Matěj
• Other Authors: Pilařová, Pavla
• Format: Dissertation
• Language: Czech
• Published: 2021
• Online Access: http://www.nusl.cz/ntk/nusl-452313

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis Candidate: Matěj Nodžák Tutor: PharmDr. Pavla Pilařová, Ph.D. Title of Thesis: Separation of Aciclovir and its Degradation Product This work investigated the retention behavior of aciclovir and its degradation product guanine using hydrophilic interaction liquid chromatography (HILIC). A column containing porous graphitized carbon (PGC) called HypercarbTM was used for analysis. The work builds on the findings of the diploma thesis of Lucie Bouzková (2016) and Lenka Teplá (2017), which dealt with the retention behavior of these analytes on a zirconium column modified with elemental carbon ZirChrom® -CARB. The effect of the presence of 0.1% acetic acid and 0.1% trifluoroacetic acid in the mobile phases on the retention of aciclovir and guanine was investigated. Furthermore, the effect of concentration of ammonium fluoride, diethylamine and phosphate buffers in mobile phases on the elution of analytes was studied. The mobile phases also contained different compositions of the organic component. In addition, different ratios between the aqueous and organic components were used. The mechanism of retention on the used column is related to many factors, is very complex and...