Studium mechanismů senzitivity a rezistence k extracelulárnímu adenosinu různých buněk \kur{Drosophila melanogaster in vitro}

• Main Author: BREJCHOVÁ, Lucie
• Format: Dissertation
• Language: Czech
• Published: 2011
• Online Access: http://www.nusl.cz/ntk/nusl-54901

Extracellular adenosine is a multi-potent signalling molecule implicated in many physiological and pathophysiological conditions. The level of adenosine signalling seems to be attenuated by te action of adenosine deaminases and adenosine uptake into the intracellular space via nucleoside transporters. From many in vitro studies on both vertebrate and invertebrate cell lines, adenosine is known to reduce cell survival. The mechanism of adenosine action seems to be complex and largely cell type specific resulting from either activation of specific adenosine receptors or adenosine uptake into cells. This study aimed to elucidate the mechanisms of adenosine toxicity in fruit fly cell lines. Experiments showed that adenosine toxicity correlated with the level of adenosine uptake, its adenosine kinase mediated phosphorylation and incorporation into cellular ATP pool. Metabolical effects of adenosine included elevation of cellular glucose level and were independent of AMP kinase activation.