Development of a Semi-synthetic Medium Supporting Adherent Growth in Coagulase-Negative Staphylococci

• Main Author: Sadeghi, Abbas
• Format: Others
• Published: PDXScholar 1992
• Subjects: Staphylococcus; Bacterial growth; Bacteria > Adhesion; Biofilms
• Online Access: https://pdxscholar.library.pdx.edu/open_access_etds/13; https://pdxscholar.library.pdx.edu/cgi/viewcontent.cgi?article=1012&context=open_access_etds

A semi-synthetic medium for use in determining adherent growth with Staphylococcus epidermidis and Staphylococcus saprophyticus was developed. Production of an adherent biofilm was dependent upon the presence of hematin in the growth medium. Clinical strains of Staphylococcus epidermidis were tested for production of an adherent biofilm in trypticase soy broth, the semi-synthetic medium and the hyperalimentary nutrient solution used in the neonatal hospital unit. An adherent biofilm was obtained when Staphylococcus epidermidis was cultured m hematin supplemented hyperalimentary solution. Growth in the hyperalimentary nutrient solution diluted with fetal calf serum showed the same growth rate as when the nutrient solution was diluted with water. The final growth yield was always higher in serum diluted nutrients. There was no effect of hematin on the growth rate of the organisms.