Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.

The protein silk fibroin (SF) from the silkworm Bombyx mori is a FDA-approved biomaterial used over centuries as sutures wire. Importantly, several evidences highlighted the potential of silk biomaterials obtained by using so-called regenerated silk fibroin (RSF) in biomedicine, tissue engineering a...

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Main Author: Sagnella, Anna <1984>
Other Authors: Bertucci, Carlo
Format: Doctoral Thesis
Language:en
Published: Alma Mater Studiorum - Università di Bologna 2015
Subjects:
Online Access:http://amsdottorato.unibo.it/6906/
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spelling ndltd-unibo.it-oai-amsdottorato.cib.unibo.it-69062015-04-16T04:54:17Z Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices. Sagnella, Anna <1984> CHIM/08 Chimica farmaceutica The protein silk fibroin (SF) from the silkworm Bombyx mori is a FDA-approved biomaterial used over centuries as sutures wire. Importantly, several evidences highlighted the potential of silk biomaterials obtained by using so-called regenerated silk fibroin (RSF) in biomedicine, tissue engineering and drug delivery. Indeed, by a water-based protocol, it is possible to obtain protein water-solution, by extraction and purification of fibroin from silk fibres. Notably, RSF can be processed in a variety of biomaterials forms used in biomedical and technological fields, displaying remarkable properties such as biocompatibility, controllable biodegradability, optical transparency, mechanical robustness. Moreover, RSF biomaterials can be doped and/or chemical functionalized with drugs, optically active molecules, growth factors and/or chemicals In this view, activities of my PhD research program were focused to standardize the process of extraction and purification of protein to get the best physical and chemical characteristics. The analysis of the chemo-physical properties of the fibroin involved both the RSF water-solution and the protein processed in film. Chemo-physical properties have been studied through: vibrational (FT-IR and Raman-FT) and optical (absorption and emission UV-VIS) spectroscopy, nuclear magnetic resonance (1H and 13C NMR), thermal analysis and thermo-gravimetric scan (DSC and TGA). In the last year of my PhD, activities were focused to study and define innovative methods of functionalization of the silk fibroin solution and films. Indeed, research program was the application of different methods of manufacturing approaches of the films of fibroin without the use of harsh treatments and organic solvents. New approaches to doping and chemical functionalization of the silk fibroin were studied. Two different methods have been identified: 1) biodoping that consists in the doping of fibroin with optically active molecules through the addition of fluorescent molecules in the standard diet used for the breeding of silkworms; 2) chemical functionalization via silylation. Alma Mater Studiorum - Università di Bologna Bertucci, Carlo 2015-04-08 Doctoral Thesis PeerReviewed application/pdf en http://amsdottorato.unibo.it/6906/ info:eu-repo/semantics/openAccess
collection NDLTD
language en
format Doctoral Thesis
sources NDLTD
topic CHIM/08 Chimica farmaceutica
spellingShingle CHIM/08 Chimica farmaceutica
Sagnella, Anna <1984>
Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
description The protein silk fibroin (SF) from the silkworm Bombyx mori is a FDA-approved biomaterial used over centuries as sutures wire. Importantly, several evidences highlighted the potential of silk biomaterials obtained by using so-called regenerated silk fibroin (RSF) in biomedicine, tissue engineering and drug delivery. Indeed, by a water-based protocol, it is possible to obtain protein water-solution, by extraction and purification of fibroin from silk fibres. Notably, RSF can be processed in a variety of biomaterials forms used in biomedical and technological fields, displaying remarkable properties such as biocompatibility, controllable biodegradability, optical transparency, mechanical robustness. Moreover, RSF biomaterials can be doped and/or chemical functionalized with drugs, optically active molecules, growth factors and/or chemicals In this view, activities of my PhD research program were focused to standardize the process of extraction and purification of protein to get the best physical and chemical characteristics. The analysis of the chemo-physical properties of the fibroin involved both the RSF water-solution and the protein processed in film. Chemo-physical properties have been studied through: vibrational (FT-IR and Raman-FT) and optical (absorption and emission UV-VIS) spectroscopy, nuclear magnetic resonance (1H and 13C NMR), thermal analysis and thermo-gravimetric scan (DSC and TGA). In the last year of my PhD, activities were focused to study and define innovative methods of functionalization of the silk fibroin solution and films. Indeed, research program was the application of different methods of manufacturing approaches of the films of fibroin without the use of harsh treatments and organic solvents. New approaches to doping and chemical functionalization of the silk fibroin were studied. Two different methods have been identified: 1) biodoping that consists in the doping of fibroin with optically active molecules through the addition of fluorescent molecules in the standard diet used for the breeding of silkworms; 2) chemical functionalization via silylation.
author2 Bertucci, Carlo
author_facet Bertucci, Carlo
Sagnella, Anna <1984>
author Sagnella, Anna <1984>
author_sort Sagnella, Anna <1984>
title Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
title_short Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
title_full Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
title_fullStr Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
title_full_unstemmed Silk Fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
title_sort silk fibroin: a biopolymer platform for innovative pharmaceutical formulation and biomedical devices.
publisher Alma Mater Studiorum - Università di Bologna
publishDate 2015
url http://amsdottorato.unibo.it/6906/
work_keys_str_mv AT sagnellaanna1984 silkfibroinabiopolymerplatformforinnovativepharmaceuticalformulationandbiomedicaldevices
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