Forensic DNA Extraction Strategies for PCR Analysis

• Main Author: Van Winkle, Carolyn
• Other Authors: Benjamin, Robert C.
• Format: Others
• Language: English
• Published: University of North Texas 1998
• Subjects: Forensic science; DNA extraction; PCR; Polymerase chain reaction.; Forensic genetics > Technique.; DNA fingerprinting.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc278269/

There is a transition nationwide on the analysis of forensic evidentiary stains containing biological material from traditional serology to Polymerase Chain Reaction (PCR) methodologies. The increased sensitivity of PCR, the limited number of alleles at each locus, and the necessity of producing unambiguous data for entry into the FBI's Combined DNA Index System make this study of extraction procedures of utmost importance. A "single tube" extraction procedure for blood stains collected onto FTA™ paper and a modified differential nonorganic extraction method from spermatozoa containing mixed stains were analyzed and compared. The extraction success was evaluated by amplification and typing of the amplified fragment length polymorphism, D1S80. These modifications of the nonorganic method utilized gave an improved separation of the spermatozoa-containing mixed stains.