Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions
The enzyme 3-Hydroxy-3- Methylglutaryl Coenzyme A Reductase catalyzes the rate limiting step of hepatic cholesterol biosynthesis and is unique among the enzymes in the early part of the pathway in that it is membrane bound. This gives rise to potential regulation of the enzyme through interactions w...
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North Texas State University
1982
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ndltd-unt.edu-info-ark-67531-metadc3305882018-04-26T05:17:12Z Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions Brent, Lynn G. (Lynn Gran) HMG-CoA reductase enzyme-lipid interactions protein-lipid interactions 3-Hydroxy-3-methylglutaryl coenzyme A reductase. Enzyme-linked immunosorbent assay. Cholesterol -- Physiological aspects. The enzyme 3-Hydroxy-3- Methylglutaryl Coenzyme A Reductase catalyzes the rate limiting step of hepatic cholesterol biosynthesis and is unique among the enzymes in the early part of the pathway in that it is membrane bound. This gives rise to potential regulation of the enzyme through interactions with the endoplasmic reticulum membrane. A purification procedure has been developed which consistently produces enzyme of high specific activity. In order to fully characterize the interactions between HMG-CoA reductase and the lipids in its immediate environment, HMG-CoA reductase was purified to homogeneity and shown to be a protein-lipid complex. North Texas State University Thompson, Richard E. Daugherty, Kenneth E. Gracy, Robert W. Cooke, J. V. Harris, Ben G. 1982-12 Thesis or Dissertation vi, 186 leaves : ill. Text local-cont-no: 1002782286-Brent call-no: 379 N81 no. 1940 untcat: b1311689 oclc: 11402322 https://digital.library.unt.edu/ark:/67531/metadc330588/ ark: ark:/67531/metadc330588 English Public Brent, Lynn G. (Lynn Gran) Copyright Copyright is held by the author, unless otherwise noted. All rights reserved. |
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English |
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Others
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HMG-CoA reductase enzyme-lipid interactions protein-lipid interactions 3-Hydroxy-3-methylglutaryl coenzyme A reductase. Enzyme-linked immunosorbent assay. Cholesterol -- Physiological aspects. |
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HMG-CoA reductase enzyme-lipid interactions protein-lipid interactions 3-Hydroxy-3-methylglutaryl coenzyme A reductase. Enzyme-linked immunosorbent assay. Cholesterol -- Physiological aspects. Brent, Lynn G. (Lynn Gran) Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
description |
The enzyme 3-Hydroxy-3- Methylglutaryl Coenzyme A Reductase catalyzes the rate limiting step of hepatic cholesterol biosynthesis and is unique among the enzymes in the early part of the pathway in that it is membrane bound. This gives rise to potential regulation of the enzyme through interactions with the endoplasmic reticulum membrane. A purification procedure has been developed which consistently produces enzyme of high specific activity. In order to fully characterize the interactions between HMG-CoA reductase and the lipids in its immediate environment, HMG-CoA reductase was purified to homogeneity and shown to be a protein-lipid complex. |
author2 |
Thompson, Richard E. |
author_facet |
Thompson, Richard E. Brent, Lynn G. (Lynn Gran) |
author |
Brent, Lynn G. (Lynn Gran) |
author_sort |
Brent, Lynn G. (Lynn Gran) |
title |
Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
title_short |
Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
title_full |
Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
title_fullStr |
Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
title_full_unstemmed |
Purification of HMG-CoA Reductase and Regulation by Protein-Lipid Interactions |
title_sort |
purification of hmg-coa reductase and regulation by protein-lipid interactions |
publisher |
North Texas State University |
publishDate |
1982 |
url |
https://digital.library.unt.edu/ark:/67531/metadc330588/ |
work_keys_str_mv |
AT brentlynnglynngran purificationofhmgcoareductaseandregulationbyproteinlipidinteractions |
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1718632739273441280 |