X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain
The response of frog sciatic nerve action potentials to x-irradiation and metabolic (carbon monoxide) or transport (ouabain) inhibition was determined in an attempt to further identify the nature of radiation insult to nervous tissue. Carbon monoxide, ouabain (2 X 10-5 M), and nitrogen anoxia were s...
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North Texas State University
1971
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ndltd-unt.edu-info-ark-67531-metadc6634002017-03-17T08:41:33Z X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain Thompson, Wesley J. carbon monoxide nitrogen inhibitions ouabain inhibition The response of frog sciatic nerve action potentials to x-irradiation and metabolic (carbon monoxide) or transport (ouabain) inhibition was determined in an attempt to further identify the nature of radiation insult to nervous tissue. Carbon monoxide, ouabain (2 X 10-5 M), and nitrogen anoxia were shown to produce a near linear decline in action potential amplitude. The carbon monoxide and nitrogen inhibitions of activity were reversible in air; the carbon monoxide inhibition was light reversible. Ouabain inhibition was partially reversible by soaking the nerve in aerated Ringer's. Application of 120 kv x-rays (75 Kr at 4.9 Kr/min) to nerves during the linear decline in spike amplitude brought about a marked enhancement (146%) of inhibition by 99% CO/l% 02, nitrogen (136%), and ouabain (265%). All bhanges were shown to be statistically significant by a regression analysis. However, x-irradiation did not appear to alter the air reversibility of carbon monoxide and nitrogen inhibitions nor the reversibility in Ringerts of the ouabain inhibition. Additionally x-irradiation completely blocked light reversal of 98% CO/2% 02 inhibition and produced a decline in activity. A possible interpretation of these results is a compensation for radiation action at this dosage requiring metabolism and ion pump activity. North Texas State University Lott, James R. Norton, S. J. Harris, Ben G. 1971-12 Thesis or Dissertation v, 58 leaves: ill. Text local-cont-no: 1002774114-Thompson call-no: 379 N81 no.4425 untcat: b2217666 https://digital.library.unt.edu/ark:/67531/metadc663400/ ark: ark:/67531/metadc663400 English Public Thompson, Wesley J. Copyright Copyright is held by the author, unless otherwise noted. All rights |
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carbon monoxide nitrogen inhibitions ouabain inhibition |
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carbon monoxide nitrogen inhibitions ouabain inhibition Thompson, Wesley J. X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
description |
The response of frog sciatic nerve action potentials to x-irradiation and metabolic (carbon monoxide) or transport (ouabain) inhibition was determined in an attempt to further identify the nature of radiation insult to nervous tissue. Carbon monoxide, ouabain (2 X 10-5 M), and nitrogen anoxia were shown to produce a near linear decline in action potential amplitude. The carbon monoxide and nitrogen inhibitions of activity were reversible in air; the carbon monoxide inhibition was light reversible. Ouabain inhibition was partially reversible by soaking the nerve in aerated Ringer's. Application of 120 kv x-rays (75 Kr at 4.9 Kr/min) to nerves during the linear decline in spike amplitude brought about a marked enhancement (146%) of inhibition by 99% CO/l% 02, nitrogen (136%), and ouabain (265%). All bhanges were shown to be statistically significant by a regression analysis.
However, x-irradiation did not appear to alter the air reversibility of carbon monoxide and nitrogen inhibitions nor the reversibility in Ringerts of the ouabain inhibition. Additionally x-irradiation completely blocked light reversal of 98% CO/2% 02 inhibition and produced a decline in activity. A possible interpretation of these results is a compensation for radiation action at this dosage requiring metabolism and ion pump activity. |
author2 |
Lott, James R. |
author_facet |
Lott, James R. Thompson, Wesley J. |
author |
Thompson, Wesley J. |
author_sort |
Thompson, Wesley J. |
title |
X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
title_short |
X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
title_full |
X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
title_fullStr |
X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
title_full_unstemmed |
X-irradiation Effects on the Action Potentials of Frog Sciatic Nerves Inhibited by Carbon Monoxide and Ouabain |
title_sort |
x-irradiation effects on the action potentials of frog sciatic nerves inhibited by carbon monoxide and ouabain |
publisher |
North Texas State University |
publishDate |
1971 |
url |
https://digital.library.unt.edu/ark:/67531/metadc663400/ |
work_keys_str_mv |
AT thompsonwesleyj xirradiationeffectsontheactionpotentialsoffrogsciaticnervesinhibitedbycarbonmonoxideandouabain |
_version_ |
1718432671306088448 |