Studies on Poly(ADP-ribose) Metabolism and Chromatin Structure

• Main Author: Cárdenas-Corona, María E. (María Elena)
• Other Authors: Jacobson, Myron
• Format: Others
• Language: English
• Published: University of North Texas 1988
• Subjects: nucleoproteins; chromatin; ribose; Nucleoproteins > Metabolism.; Chromatin.; Ribose.
• Online Access: https://digital.library.unt.edu/ark:/67531/metadc798380/

In these studies, a procedure which allowed the in vivo labeling and detection of poly(ADP-ribose) was combined with nuclear fractionation techniques to analyze the nuclear distribution of ADP-ribose polymers. The results from these studies suggest the occurrence of poly(ADP-ribose) metabolism in two compartments of chromatin; one that is nuclear matrix-associated and one that is not. The biological significance of this compartmentalization is conceptualization in a model. This model postulates that, under some physiological conditions, poly(ADP-ribose) metabolism accomplishes the reversible targeting of specific regions of chromatin to the nuclear matrix domain by modulating DNA-protein and or protein-protein interactions.