Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers
The nanoscale manipulation of soft-matter, such as biological tissues, in its native environment has promising applications in medicine to correct for defects (eg. eye cataracts) or to destroy malignant regions (eg. cancerous tumours). To achieve this we need the ability to first image and then do p...
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ndltd-uottawa.ca-oai-ruor.uottawa.ca-10393-200622018-01-05T19:00:58Z Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers Guay, Jean-Michel Bhardwaj, Ravi V. Multiphoton Microscopy Interaction of intense pulses with polymers light-matter interaction The nanoscale manipulation of soft-matter, such as biological tissues, in its native environment has promising applications in medicine to correct for defects (eg. eye cataracts) or to destroy malignant regions (eg. cancerous tumours). To achieve this we need the ability to first image and then do precise ablation with sub-micron resolution with the same setup. For this purpose, we designed and built a multiphoton microscope and tested it on goldfish gills and bovine cells. We then studied light-matter interaction on a hard polymer (PMMA) because the nature of ablation of soft-matter in its native environment is complex and not well understood. Ablation and modification thresholds for successive laser shots were obtained. The ablation craters revealed 3D nanostructures and polarization dependent orientation. The interaction also induced localized porosity in PMMA that can be controlled. 2011-06-20T18:47:43Z 2011-06-20T18:47:43Z 2011 2011 Thesis http://hdl.handle.net/10393/20062 http://dx.doi.org/10.20381/ruor-4651 en Université d'Ottawa / University of Ottawa |
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en |
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Multiphoton Microscopy Interaction of intense pulses with polymers light-matter interaction |
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Multiphoton Microscopy Interaction of intense pulses with polymers light-matter interaction Guay, Jean-Michel Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
description |
The nanoscale manipulation of soft-matter, such as biological tissues, in its native environment has promising applications in medicine to correct for defects (eg. eye cataracts) or to destroy malignant regions (eg. cancerous tumours). To achieve this we need the ability to first image and then do precise ablation with sub-micron resolution with the same setup. For this purpose, we designed and built a multiphoton microscope and tested it on goldfish gills and bovine cells. We then studied light-matter interaction on a hard polymer (PMMA) because the nature of ablation of soft-matter in its native environment is complex and not well understood. Ablation and modification thresholds for successive laser shots were obtained. The ablation craters revealed 3D nanostructures and polarization dependent orientation. The interaction also induced localized porosity in PMMA that can be controlled. |
author2 |
Bhardwaj, Ravi V. |
author_facet |
Bhardwaj, Ravi V. Guay, Jean-Michel |
author |
Guay, Jean-Michel |
author_sort |
Guay, Jean-Michel |
title |
Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
title_short |
Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
title_full |
Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
title_fullStr |
Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
title_full_unstemmed |
Multiphoton Microscopy and Interaction of Intense Light Pulses with Polymers |
title_sort |
multiphoton microscopy and interaction of intense light pulses with polymers |
publisher |
Université d'Ottawa / University of Ottawa |
publishDate |
2011 |
url |
http://hdl.handle.net/10393/20062 http://dx.doi.org/10.20381/ruor-4651 |
work_keys_str_mv |
AT guayjeanmichel multiphotonmicroscopyandinteractionofintenselightpulseswithpolymers |
_version_ |
1718597322530619392 |