Expression of Foreign Genes in the Pseudomonas Bacteriophage Pf3
Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. It was hypothesized that expression of foreign genes would be possible using the phage’s promoters. This hypothesis was tested...
| Main Author: | |
|---|---|
| Format: | Others |
| Published: |
VCU Scholars Compass
2012
|
| Subjects: | |
| Online Access: | http://scholarscompass.vcu.edu/etd/357 http://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=1356&context=etd |
| Summary: | Filamentous bacteriophages were engineered to express foreign genes with the ultimate purpose of displaying transmission control anti-malarial peptides as in phage display. It was hypothesized that expression of foreign genes would be possible using the phage’s promoters. This hypothesis was tested by assuming that promoters for the phage major coat protein (MCP) gene would also promote the expression of any foreign gene inserted downstream of the MCP gene. As proof of principle, the bacteriophages Pf3, Pf1, and M13 were engineered in this way to successfully synthesize Enhanced Green Fluorescent Protein (EGFP). Type 88 phage display on the EGFP recombinant Pf3 was attempted by fusing a second copy of its MCP gene to the existing EGFP gene. This resulted in a phage display Pf3 replacement vector which was then used to construct a phage for displaying an anti-malarial peptide. |
|---|