The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River
Antibiotic resistance is a major threat to human health. Clinical situations are the main focus for antibiotic resistance research, but understanding the spread of resistance in the environment is also vital. A major contributor to this spread is wastewater from combined sewer overflow (CSO) events....
Main Author: | |
---|---|
Format: | Others |
Published: |
VCU Scholars Compass
2017
|
Subjects: | |
Online Access: | https://scholarscompass.vcu.edu/etd/5179 https://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=6267&context=etd |
id |
ndltd-vcu.edu-oai-scholarscompass.vcu.edu-etd-6267 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-vcu.edu-oai-scholarscompass.vcu.edu-etd-62672019-10-20T22:03:45Z The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River Levengood, Enjolie Antibiotic resistance is a major threat to human health. Clinical situations are the main focus for antibiotic resistance research, but understanding the spread of resistance in the environment is also vital. A major contributor to this spread is wastewater from combined sewer overflow (CSO) events. The effect of CSO events on antibiotic resistance in the James River near Richmond, Virginia was studied using genomic and microbiological approaches. The abundance of genes associated with resistance to quinolones (qnrA) and tetracycline (tetW) was strongly correlated with the presence of fecal indicator bacteria (E. coli abundance) as well as total nitrogen and phosphorus loads, which suggests an anthropogenic source of these genes. Abundance of the blaTEM gene, which confers resistance to β-lactam antibiotics, was elevated during CSO events and increased with precipitation and river discharge. Bacteria isolated during a CSO event were resistant to more antibiotics and had higher multi-drug resistance when compared to isolates from a non-event. This study demonstrated that CSO events are contributing to the spread of antibiotic resistance. 2017-01-01T08:00:00Z text application/pdf https://scholarscompass.vcu.edu/etd/5179 https://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=6267&context=etd © Enjolie Levengood Theses and Dissertations VCU Scholars Compass combined sewer overflow CSO antibiotic resistance James River antibiotic resistance gene |
collection |
NDLTD |
format |
Others
|
sources |
NDLTD |
topic |
combined sewer overflow CSO antibiotic resistance James River antibiotic resistance gene |
spellingShingle |
combined sewer overflow CSO antibiotic resistance James River antibiotic resistance gene Levengood, Enjolie The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
description |
Antibiotic resistance is a major threat to human health. Clinical situations are the main focus for antibiotic resistance research, but understanding the spread of resistance in the environment is also vital. A major contributor to this spread is wastewater from combined sewer overflow (CSO) events. The effect of CSO events on antibiotic resistance in the James River near Richmond, Virginia was studied using genomic and microbiological approaches. The abundance of genes associated with resistance to quinolones (qnrA) and tetracycline (tetW) was strongly correlated with the presence of fecal indicator bacteria (E. coli abundance) as well as total nitrogen and phosphorus loads, which suggests an anthropogenic source of these genes. Abundance of the blaTEM gene, which confers resistance to β-lactam antibiotics, was elevated during CSO events and increased with precipitation and river discharge. Bacteria isolated during a CSO event were resistant to more antibiotics and had higher multi-drug resistance when compared to isolates from a non-event. This study demonstrated that CSO events are contributing to the spread of antibiotic resistance. |
author |
Levengood, Enjolie |
author_facet |
Levengood, Enjolie |
author_sort |
Levengood, Enjolie |
title |
The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
title_short |
The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
title_full |
The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
title_fullStr |
The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
title_full_unstemmed |
The effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the James River |
title_sort |
effect of combined sewer overflows on the abundance of antibiotic resistance genes and bacteria in the james river |
publisher |
VCU Scholars Compass |
publishDate |
2017 |
url |
https://scholarscompass.vcu.edu/etd/5179 https://scholarscompass.vcu.edu/cgi/viewcontent.cgi?article=6267&context=etd |
work_keys_str_mv |
AT levengoodenjolie theeffectofcombinedseweroverflowsontheabundanceofantibioticresistancegenesandbacteriainthejamesriver AT levengoodenjolie effectofcombinedseweroverflowsontheabundanceofantibioticresistancegenesandbacteriainthejamesriver |
_version_ |
1719272723540082688 |