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02963nam a2200529Ia 4500 |
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0.1016-j.bios.2022.114203 |
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220421s2022 CNT 000 0 und d |
| 020 |
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|a 09565663 (ISSN)
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|a Molecularly imprinted polymer-based extended-gate field-effect transistor (EG-FET) chemosensor for selective determination of matrix metalloproteinase-1 (MMP-1) protein
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| 260 |
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|b Elsevier Ltd
|c 2022
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| 856 |
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|z View Fulltext in Publisher
|u https://doi.org/10.1016/j.bios.2022.114203
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|a A conducting molecularly imprinted polymer (MIP) film was integrated with an extended-gate field-effect transistor (EG-FET) transducer to determine epitopes of matrix metalloproteinase-1 (MMP-1) protein biomarker of idiopathic pulmonary fibrosis (IPF) selectively. Most suitable epitopes for imprinting were selected with Basic Local Alignment Search Tool software. From a pool of MMP-1 epitopes, the two, i.e., MIAHDFPGIGHK and HGYPKDIYSS, the relatively short ones, most promising for MMP-1 determination, were selected, mainly considering their advantageous outermost location in the protein molecule and stability against aggregation. MIPs templated with selected epitopes of the MMP-1 protein were successfully prepared by potentiodynamic electropolymerization and simultaneously deposited as thin films on electrodes. The chemosensors, constructed of MIP films integrated with EG-FET, proved useful in determining these epitopes even in a medium as complex as a control serum. The limit of detection for the MIAHDFPGIGHK and HGYPKDIYSS epitope was ∼60 and 20 nM, respectively. Moreover, the chemosensors selectively recognized whole MMP-1 protein in the 50–500 nM concentration range in buffered control serum samples. © 2022 The Authors
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|a BLAST
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|a BLAST
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|a Chemosensor
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|a Chemosensor
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|a Chemo-sensors
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|a EG-FET
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|a Electropolymerization
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|a Epitope imprinting
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|a Epitope imprinting
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|a Epitopes
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|a Extended-gate field-effect transistor
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|a Extended-gate field-effect transistors
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|a Field effect transistors
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|a Idiopathic pulmonary fibrosis
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|a Idiopathic pulmonary fibrosis
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|a IPF
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|a Matrix metalloproteinase-1
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|a Matrix metalloproteinase-1 protein
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|a MIP
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|a MMP-1 protein
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|a Molecularly imprinted polymer
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|a Molecularly Imprinted Polymer
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|a Proteins
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|a Thin film circuits
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|a Thin films
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|a Bartold, K.
|e author
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|a Borowicz, P.
|e author
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|a Iskierko, Z.
|e author
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|a Kalecki, J.
|e author
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|a Kutner, W.
|e author
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|a Lin, C.-Y.
|e author
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|a Lin, H.-Y.
|e author
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|a Noworyta, K.
|e author
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|a Sharma, P.S.
|e author
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| 773 |
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|t Biosensors and Bioelectronics
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