An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines

The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed...

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Bibliographic Details
Main Authors: Clark, K.J (Author), Ekker, S.C (Author), Kar, B. (Author), Restrepo-Castillo, S. (Author), Sabharwal, A. (Author), Simone, B.W (Author)
Format: Article
Language:English
Published: Cell Press 2022
Subjects:
Cell Biology
CRISPR
Genetics
Molecular Biology
Sequencing
Online Access:View Fulltext in Publisher
Description
Summary:The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed to be easily modified to work in diverse applications where editing of mitochondrial DNA is desired. For complete details on the use and execution of this protocol, please refer to Sabharwal et al. (2021) and Ma et al. (2016). © 2022 The Author(s)
ISBN:26661667 (ISSN)
DOI:10.1016/j.xpro.2022.101288

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