An optimized FusX assembly-based technique to introduce mitochondrial TC-to-TT variations in human cell lines
The FusX TALE Based Editor (FusXTBE) is a programmable base editing platform that can introduce specific TC-to-TT variations in the mitochondrial DNA (mtDNA). Here, we provide a protocol describing the synthesis and testing of the FusXTBE plasmids in cultured human cell lines. This tool is designed...
Main Authors: | Clark, K.J (Author), Ekker, S.C (Author), Kar, B. (Author), Restrepo-Castillo, S. (Author), Sabharwal, A. (Author), Simone, B.W (Author) |
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Format: | Article |
Language: | English |
Published: |
Cell Press
2022
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Subjects: | |
Online Access: | View Fulltext in Publisher |
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