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10-1093-stmcls-sxac001 |
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|a 15494918 (ISSN)
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|a Hematopoietic Stem Cell-Independent Differentiation of Mast Cells From Mouse Intraembryonic VE-Cadherin+ Cells
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|b NLM (Medline)
|c 2022
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|a 14
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|z View Fulltext in Publisher
|u https://doi.org/10.1093/stmcls/sxac001
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|a Hematopoietic stem cell (HSC)-independent hematopoiesis from hemogenic endothelial cells (HECs) in the mouse embryo has been recognized as a source of tissue-resident hematopoietic cells in adult mice. Connective tissue mast cells (MCs) have been reported to originate from VE-cadherin (VE-cad)-expressing HECs in the yolk sac and embryo proper (EP) by a VE-cad-Cre-mediated lineage-tracing analysis. However, it remains unclear whether MCs are generated via a conventional HSC-dependent hematopoietic differentiation pathway, or whether through a fast-track pathway bypassing the emergence of HSCs. Here, we investigated whether EP-derived VE-cad+ cells differentiate into MCs independently of HSCs. VE-cad+ cells isolated from the embryonic day (E) 9.5-10.5 EP robustly formed connective tissue-type MCs in a newly established co-culture system using PA6 stromal cells. In contrast, bone marrow (BM) reconstitution assays of cultured cells indicated that E9.5 VE-cad+ cells did not differentiate into transplantable HSCs in this culture condition. Lymphoid-biased HSCs with a limited self-renewal capacity were occasionally detected in some cultures of E10.5 VE-cad+ cells, while MC growth was constantly observed in all cultures examined. HSCs purified from adult BM required a more extended culture period to form MCs in the PA6 co-culture than the embryonic VE-cad+ cells. Furthermore, E9.5-E10.5 VE-cad+ cells contributed to tissue-resident MCs in postnatal mice when transplanted into the peritoneal cavity of newborn mice. These results suggest that EP-derived VE-cad+ cells generate MCs independently of HSC development in vitro and possess the potential of generating connective tissue MCs in vivo, although the exact differentiation program remains unsolved. © The Author(s) 2022. Published by Oxford University Press. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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|a animal
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|a Animals
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|a Antigens, CD
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|a cadherin
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|a Cadherins
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|a hemangioblast
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|a Hemangioblasts
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|a hematopoiesis
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|a Hematopoiesis
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|a hematopoietic stem cell
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|a hematopoietic stem cells
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|a Hematopoietic Stem Cells
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|a hemogenic endothelial cells
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|a leukocyte antigen
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|a mast cell
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|a mast cells
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|a Mast Cells
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|a metabolism
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|a Mice
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|a mouse
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|a mouse
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|a stromal cells
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|a vascular endothelial cadherin
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|a VE-cadherin
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|a Morino-Koga, S.
|e author
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|a Ogawa, M.
|e author
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|a Tsuruda, M.
|e author
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|t Stem cells (Dayton, Ohio)
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