Label-free monitoring of spatiotemporal changes in the stem cell cytoskeletons in time-lapse phase-contrast microscopy
Investigation of the dynamic structural changes in the actin cytoskeleton during cell migration provides crucial information about the physiological conditions of a stem cell during in-vitro culture. Here we proposed a quantitative analytical model associated with texture extraction with cell tracki...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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Optica Publishing Group (formerly OSA)
2022
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| Subjects: | |
| Online Access: | View Fulltext in Publisher |
| Summary: | Investigation of the dynamic structural changes in the actin cytoskeleton during cell migration provides crucial information about the physiological conditions of a stem cell during in-vitro culture. Here we proposed a quantitative analytical model associated with texture extraction with cell tracking techniques for in situ monitoring of the cytoskeletal density change of stem cells in phase-contrast microscopy without fluorescence staining. The reliability of the model in quantifying the texture density with different orientation was first validated using a series of simulated textural images. The capability of the method to reflect the spatiotemporal regulation of the cytoskeletal structure of a living stem cell was further proved by applying it to a set of 72 h phase-contrast microscopic video of the growth dynamics of mesenchymal stem cells in vitro culture. © 2022 Optica Publishing Group under the terms of the Optica Open Access Publishing Agreement |
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| Physical Description: | 11 |
| ISBN: | 21567085 (ISSN) |
| DOI: | 10.1364/BOE.452822 |