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10-3390-antibiotics11040444 |
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|a 20796382 (ISSN)
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|a Antimicrobial Resistance in Escherichia coli from the Broiler Farm Environment, with Detection of SHV-12-Producing Isolates
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|b MDPI
|c 2022
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|z View Fulltext in Publisher
|u https://doi.org/10.3390/antibiotics11040444
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|a Antimicrobial resistance is an important One Health challenge that encompasses the human, animal, and environmental fields. A total of 111 Escherichia coli isolates previously recovered from manure (n = 57) and indoor air (n = 54) samples from a broiler farm were analyzed to determine their phenotypes and genotypes of antimicrobial resistance and integron characterization; in addition, plas-mid replicon analysis and molecular typing were performed in extended-spectrum-beta-lactamase (ESBL) producer isolates. A multidrug-resistance phenotype was detected in 46.8% of the isolates, and the highest rates of resistance were found for ampicillin, trimethoprim–sulfamethoxazole, and tetracycline (>40%); moreover, 15 isolates (13.5%) showed susceptibility to all tested antibiotics. None of the isolates showed imipenem and/or cefoxitin resistance. Twenty-three of the one hundred and eleven E. coli isolates (20.7%) were ESBL producers and carried the blaSHV-12 gene; one of these isolates was recovered from the air, and the remaining 22 were from manure samples. Most of ESBL-positive isolates carried the cmlA (n = 23), tet(A) (n = 19), and aac(6′)-Ib-cr (n = 11) genes. The following genetic lineages were identified among the ESBL-producing isolates (sequence type-phylogroup-clonotype): ST770-E-CH116–552 (n = 12), ST117-B2-CH45–97 (n = 4), ST68-E-CH26–382/49 (n = 3), ST68-E-CH26–49 (n = 1), and ST10992-A/B1-CH11–23/41/580 (n = 4); the latter two were detected for the first time in the poultry sector. At least two plasmid replicon types were detected in the ESBL-producing E. coli isolates, with IncF, IncF1B, IncK, and IncHI1 being the most frequently found. The following antimicrobial resistance genes were identified among the non-ESBL-producing isolates (number of isolates): blaTEM (58), aac(6′)-Ib-cr (6), qnrS (2), aac(3)-II (2), cmlA (6), tet(A)/tet(B) (22), and sul1/2/3 (51). Four different gene-cassette arrays were detected in the variable region of class 1 (dfrA1-aadA1, dfrA12-aadA2, and dfrA12-orf-aadA2-cmlA) and class 2 integrons (sat2-aadA1-orfX). This work reveals the worrying presence of antimicrobial-resistant E. coli in the broiler farm environment, with ESBL-producing isolates of SHV-12 type being extensively disseminated. © 2022 by the authors. Licensee MDPI, Basel, Switzerland.
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|a amino acid substitution
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|a amoxicillin plus clavulanic acid
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|a ampicillin
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|a antibiotic resistance
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|a antibiotic sensitivity
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|a Article
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|a beta lactamase SHV
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|a beta lactamase SHV 12
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|a broiler
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|a broiler farm
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|a cefotaxime
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|a cefoxitin
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|a ceftazidime
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|a chloramphenicol
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|a ciprofloxacin
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|a coliform bacterium
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|a colistin
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|a cotrimoxazole
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|a DNA extraction
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|a DNA sequence
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|a environmental
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|a ESBL
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|a Escherichia coli
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|a Escherichia coli
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|a extended spectrum beta lactamase
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|a farm animal
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|a gene cassette
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|a genotyping
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|a gentamicin
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|a imipenem
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|a integron
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|a matrix assisted laser desorption ionization time of flight mass spectrometry
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|a minimum inhibitory concentration
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|a molecular typing
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|a multidrug resistance
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|a multilocus sequence typing
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|a nonhuman
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|a phenotype
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|a plasmid
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|a point mutation
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|a polymerase chain reaction
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|a poultry manure
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|a replicon
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|a SHV-12
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|a tetracycline
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|a unclassified drug
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|a Campaña-Burguet, A.
|e author
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|a Carvalho, I.
|e author
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|a Fernández-Fernández, R.
|e author
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|a Hidalgo-Sanz, R.
|e author
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|a Latorre-Fernández, J.
|e author
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|a Martínez-álvarez, S.
|e author
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|a Olarte, C.
|e author
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|a Sanz, S.
|e author
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|a Torres, C.
|e author
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|a Zarazaga, M.
|e author
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|t Antibiotics
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