Transcription factor FOXA3 promotes the development of Hepatoblastoma via regulating HNF1A, AFP, and ZFHX3 expression

• Main Authors: Zhan, X. (Author), Zhao, A. (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: AFP; AFP protein, human; alpha fetoprotein; alpha-Fetoproteins; Article; cancer tissue; cell assay; cell cloning; cell protein; cell survival; Cell Survival; cell viability; child; Child, Preschool; clinical article; colony formation; controlled study; down regulation; female; Female; FOXA3; FOXA3 protein, human; gene control; gene expression; gene knockdown; genetics; hepatoblastoma; Hepatoblastoma; hepatoblastoma cell line; hepatocyte nuclear factor 1alpha; Hepatocyte Nuclear Factor 1-alpha; hepatocyte nuclear factor 3gamma; Hepatocyte Nuclear Factor 3-gamma; HNF1A; HNF1A protein, human; homeodomain protein; Homeodomain Proteins; human; human cell; human tissue; Humans; immunohistochemistry; infant; Infant; Liver Neoplasms; liver tumor; male; Male; metabolism; Myc protein; pathology; preschool child; protein expression; transcription; unclassified drug; upregulation; Western blotting; ZFHX3 protein; ZFHX3 protein, human
• Online Access: View Fulltext in Publisher
• ISBN: 08878013 (ISSN)
• DOI: 10.1002/jcla.23686

Objective: In this research paper, we aimed to study the role of FOXA3 in hepatoblastoma (HB) and the molecular mechanism. Methods: Immunohistochemistry was applied to determine the expression situation of FOXA3 and AFP in HB tissues and the adjacent normal tissues. FOXA3, HNF1A, and ZFHX3 expressions in HB tissues and the normal tissues were measured by Western blot. HB cell lines were randomly divided into 4 groups: Model, si-NC, si-FOXA3-1, and si-FOXA3-2 group. The HB cell viability and colony formation characteristics in the 4 groups were explored by CCK-8 and cell cloning formation assay, respectively. The expression of FOXA3, AFP, HNF1A, ZFHX3, and MYC in HB cells after knockdown of FOXA3 was measured. Results: FOXA3, AFP, and HNF1A expressions were significantly up-regulated in HB tissues, while ZFHX3 expression was down-regulated. Knockdown of FOXA3 markedly inhibited HB cell viability and cloning formation ability. Knockdown of FOXA3 decreased FOXA3, AFP, and HNF1A/MYC expression, while increased ZFHX3 expression. Conclusion: FOXA3 promotes the occurrence and development of HB by up-regulating AFP and HNF1A/MYC expression, and down-regulating ZFHX3 expression. © 2020 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC