The clinical significance of vitamin D levels and vitamin D receptor mRNA expression in colorectal neoplasms

• Main Authors: Ding, X. (Author), Fang, Y. (Author), Huang, J. (Author), Song, H. (Author), Zhou, J. (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: 25 hydroxyvitamin D; adenocarcinoma; Adenocarcinoma; adenoma; Adenoma; adult; Article; blood; calcitriol receptor; cancer growth; cancer risk; cancer tissue; carcinogenesis; case control study; colon; Colon; colorectal adenocarcinoma; colorectal adenoma; colorectal carcinoma; Colorectal Neoplasms; colorectal tumor; controlled study; enzyme linked immunosorbent assay; female; Female; fluorescence quantitative polymerase chain reaction; genetics; human; human tissue; Humans; major clinical study; male; Male; messenger RNA; metabolism; middle aged; Middle Aged; mRNA expression level; Receptors, Calcitriol; RNA, Messenger; VDR protein, human; vitamin blood level; vitamin D; Vitamin D; vitamin D receptor
• Online Access: View Fulltext in Publisher

 Background/Aim: This study aimed to investigate the clinical significance of changes in vitamin D [25(OH)D] levels and vitamin D receptor (VDR) mRNA expression in colorectal adenoma development. Methods: Plasma concentrations of 25(OH)D and mRNA expression of VDR in tissues were determined by enzyme-linked immunosorbent assay (ELISA) and real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), respectively. In addition, the concentration of plasma 25(OH)D and levels of VDR mRNA in tissues were compared among healthy individuals and adenoma and adenocarcinoma patients. Results: Vitamin D receptor expression in colorectal adenocarcinoma tissues was significantly lower than that in para-cancerous tissues that were >5 cm away from malignant tumor sites (p < 0.01). The level of VDR expression in normal colorectal tissues from healthy individuals was significantly higher than that in colorectal adenomas (p < 0.01) and colorectal adenocarcinomas (p < 0.01); however, the VDR expression was not significantly different between colorectal adenomas and colorectal adenocarcinomas (p = 0.106). The concentration of 25(OH)D in healthy individuals was significantly higher than that in patients with colorectal adenomas (p < 0.01) and colorectal adenocarcinomas (p < 0.01); however, the concentration of 25(OH)D was not significantly different between colorectal adenomas and colorectal adenocarcinomas (p = 0.489). A low concentration of 25(OH)D was considered a risk factor for colorectal adenoma and colorectal adenocarcinoma, with odds ratios of 4.875 and 2.925, respectively. Conclusions: The 25(OH)D levels and VDR mRNA expression might be associated with the development of colorectal adenoma and its progression to adenocarcinoma. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.