Characterization of LncRNA expression profile and identification of functional LncRNAs associated with unstable angina

• Main Authors: Gu, X. (Author), Hou, J. (Author), Liu, S. (Author), Weng, R. (Author), Zhong, Z. (Author)
• Format: Article
• Language: English
• Published: John Wiley and Sons Inc 2021
• Subjects: adult; aged; Aged; Angina, Unstable; Article; biological marker; biomarker; Biomarkers; blood; controlled study; diagnostic value; disease association; down regulation; expression profile; female; Female; gene expression profiling; genetics; human; Humans; long non coding RNA DANCR; long non coding RNA FAM157C; long non coding RNA LINC01506; long non coding RNA LNC 000088; long non coding RNA LNC 000226; long non coding RNA LNC 000914; long non coding RNA LNC 000995; long non coding RNA LNC 001165; long non coding RNA LNC 001226; long non coding RNA LNC 001287; long non coding RNA LNC 001302; long non coding RNA LNC 001526; long non coding RNA LNC 002091; long non coding RNA LNC 002772; long non coding RNA MALAT1; long non coding RNA RN7SL471P; long non coding RNA RP1 167A14.2; long non coding RNA RP11 160E2.6; long non coding RNA RP11 185E8.1; long non coding RNA RP11 360I2.1; long non coding RNA RP11 734I18.1; long noncoding RNA; long untranslated RNA; major clinical study; MALAT1; male; Male; metabolism; middle aged; Middle Aged; receiver operating characteristic; RNA, Long Noncoding; transcriptome; Transcriptome; unclassified drug; unstable angina; unstable angina pectoris; upregulation
• Online Access: View Fulltext in Publisher

 Background: Increasing evidences suggest that long noncoding RNAs (lncRNAs) play critical roles in the pathogenesis of coronary artery disease (CAD). However, the association between lncRNAs expression profiles and unstable angina (UA) remained poorly known. Thus, the present study aims to investigate expression patterns, biological functions, and diagnostic value of lncRNAs in UA. Methods: The present study explored the lncRNA and mRNA expression profiles in peripheral blood mononuclear cells (PBMCs) of UA patients and normal coronary artery (NCA) controls using RNA-seq. The biological function of differentially expressed lncRNAs was analyzed using gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. The expression of the selected lncRNAs was validated in another 44 UA patients and 46 NCA controls. Receiver operating characteristic curve (ROC) was performed to evaluate the diagnostic value of lncRNAs for UA. Results: A total of 98 lncRNAs and 615 mRNAs were observed differentially expressed in PBMCs of UA patients as compared to NCA controls. The 10 most upregulated lncRNAs were LNC_000226, DANCR, RP1-167A14.2, LNC_002091, LNC_001526, LNC_001165, LNC_002772, LNC_000088, LNC_001226, and FAM157C, and the 10 most downregulated lncRNAs were RP11-734I18.1, RP11-185E8.1, RP11-360I2.1, LNC_001302, LNC_001287, RN7SL471P, LNC_000914, LINC01506, RP11-160E2.6, and LNC_000995. LNC_000226 and MALAT1 have high area under the curve values (AUC) for distinguishing UA from NCA patients (0.810 and 0.799, respectively), and the combination of MALAT1 and LNC_000226 increased the AUC value to 0.878. Conclusions: The present study added our understanding about the lncRNA expression profile in UA patients and provided potential biomarkers for the diagnosis of UA. © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.